Cell Yield Increase
Cell Yield Increase - Related Videos
04:52
Increasing cDNA Yields from Single-cell Quantities of mRNA in Standard Laboratory Reverse Transcriptase Reactions using Acoustic Microstreaming
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Cited by 11 •
2011
We describe a novel method for increasing cDNA yield from single-cell quantities of mRNA in otherwise standard laboratory reverse transcription reactions. The novelty resides in the use of a micromixer, which utilizes the phenomenon of acoustic microstreaming, to mix fluids at microliter scales more effectively than shaking, vortexing or trituration.
11:36
A RAPID Method for Blood Processing to Increase the Yield of Plasma Peptide Levels in Human Blood
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Cited by 2 •
2016
The RAPID blood processing method can be used in humans and yields higher peptide levels as well as allows for assessment of the correct molecular form. Therefore, this method will be a valuable tool in peptide research.
01:31
ATP Yield
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2019
Cellular respiration produces 30 - 32 ATP per glucose molecule. Although most of the ATP results from oxidative phosphorylation and the electron transport chain (ETC), 4 ATP are gained beforehand (2 from glycolysis and 2 from the citric acid cycle). The ETC is embedded in the inner mitochondrial membrane and is comprised of four main protein complexes and an ATP synthase. NADH and FADH2 pass electrons to these complexes, which pump protons into the intermembrane space. This distribution of...
02:22
Reaction Yield
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2020
The theoretical yield of a reaction is the amount of product estimated to form based on the stoichiometry of the balanced chemical equation. The theoretical yield assumes the complete conversion of the limiting reactant into the desired product. The amount of product that is obtained by performing the reaction is called the actual yield, and it may be less than or (very rarely) equal to the theoretical yield. Percent Yield In the case of chemical reactions, the actual yield of the product is...
09:16
A High Yield and Cost-efficient Expression System of Human Granzymes in Mammalian Cells
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Cited by 13 •
2015
We describe here a cost-efficient granzyme expression system using HEK293T cells that produces high yields of pure, fully glycosylated and enzymatically active protease.
FAQs
These conditions regulate whether cells continue growing and remain viable. Adequate nutrient availability supports cellular activity, while suitable temperature and pH help maintain growth conditions. Oxygen supply can also affect the culture environment, especially when cells are actively proliferating. Adjusting these variables together can improve production while preserving the intended characteristics of the cells.
A larger cell population or greater biomass is useful only when the cells retain the characteristics required by the biological system. Optimization therefore considers both production and cellular quality rather than yield alone. This is important for experiments, tissue models, therapeutic development, and biologics production, where changes in the cells could affect the relevance of the resulting material.
Systematic optimization examines how culture conditions affect cell growth, survival, and metabolism. By relating changes in nutrient availability, oxygen supply, pH, temperature, culture duration, or waste removal to the resulting output, researchers can identify factors that control cellular performance. This provides biological insight in addition to producing more cells or biomass for downstream work.
Begin with the defined biological system and identify the conditions most likely to limit growth or survival. Optimization can then address nutrient availability, temperature, pH, oxygen supply, culture duration, and waste removal. Evaluating these factors in relation to cell number, biomass, and desired characteristics helps determine which conditions support a useful increase rather than simply a larger culture.
The appropriate target depends on the purpose of the biological system. Cell number may be the relevant outcome when experiments or tissue models require more individual cells, whereas biomass may be more useful when the goal is to obtain greater total cellular material. Defining the intended application first helps researchers choose the yield measure that best represents success.
Higher yields can provide more material for biological experiments and tissue models, and they can support therapeutic development and production of biologics. In biotechnology and biomanufacturing, optimizing growth conditions may improve the efficiency of producing the desired cellular material. The same process also helps biology researchers study how metabolism and growth respond to environmental conditions.