Organoid Infection Model
Organoid Infection Model - Related Videos
02:47
Developing an In Vitro Model of Zika Virus Infection in Cerebral Organoids
0 Views •
2025
This video demonstrates the establishment of an in vitro model of Zika virus infection in a developing brain. Human cerebral organoids containing rosettes of stem cells, neural progenitor cells (NPCs), and neurons are infected with the Zika virus. The virus infects NPCs and triggers apoptosis, disrupting organoid development.
03:30
Establishing an Infection Model Through Microinjection of Gastric Organoids with Pathogenic Bacteria
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2025
This video demonstrates a method to establish a gastric organoid infection model by microinjecting gastric pathogen Helicobacter pylori into the lumen of the organoids. This model helps in studying infection-induced changes in gastric cells.
02:33
A Technique to Develop a Parasite Infection in Intestinal Organoids via Microinjection
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2025
This video demonstrates a microinjection technique to develop a human intestinal organoid infection model. The infectious sporozoite stage of the parasite Cryptosporidium parvum is microinjected into the organoid lumen. Upon invading the epithelial cells of the organoid, it undergoes asexual and sexual reproduction to complete its life cycle and spreads the infection.
10:30
Organoids as Model for Infectious Diseases: Culture of Human and Murine Stomach Organoids and Microinjection of Helicobacter Pylori
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Cited by 144 •
2015
Stem cell derived cultures harbor tremendous potential to model infectious diseases. Here, the culture of mouse and human gastric organoids derived from adult stem cells is described. The organoids are microinjected with the gastric pathogen Helicobacter pylori.
04:15
Bladder Tumor Organoid Model: A Method for Orthotopic Transplantation of Bladder Organoids into Recipient Murine Bladder
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2023
In this video, we describe the orthotopic transplantation of cultured bladder tumor organoids into the murine bladder to study the role of the organ environment on developing tumors.
FAQs
Organoid infection models preserve organized tissue features and multiple differentiated cell types, allowing pathogens to interact with more than a single cell population. This organization helps investigators examine pathogen entry, replication, host immune signaling, and tissue damage within one experimental system. The resulting measurements connect cellular infection with tissue-level responses.
Three-dimensional organization can provide biological context that two-dimensional cultures do not reproduce as readily. Because cells self-organize into tissue-like structures, the model can capture interactions among differentiated cell types during infection. This makes it a complementary system for studying how a pathogen affects tissue responses, rather than only measuring behavior in isolated or simplified cell layers.
Readouts can follow several stages and consequences of infection, including pathogen entry, pathogen replication, host immune signaling, tissue damage, and response to treatment. Examining these outcomes together helps researchers evaluate how infection progresses and how effectively a treatment changes pathogen-associated effects or tissue injury under controlled conditions.
A typical experimental workflow begins by establishing organoids from stem cells or tissue samples. Researchers then introduce a selected virus, bacterium, or parasite under controlled conditions and measure infection-related outcomes. This sequence links the biological starting material to observed pathogen effects, host responses, tissue damage, and treatment efficacy, allowing the study to remain focused and comparable.
Starting material determines what biological context the experiment can represent. Organoids may be derived from stem cells or tissue samples, and the latter can enable patient-specific investigations. Researchers can then introduce a virus, bacterium, or parasite into that system, linking pathogen behavior and host responses to the selected cellular context.
Researchers can apply organoid infection models to study pathogen entry and replication, characterize host immune signaling, assess tissue damage, and evaluate treatment efficacy. Their patient-specific potential is especially relevant when biological responses may vary among individuals. In addition, they provide a laboratory complement to animal studies and may help reduce reliance on animal experiments.