Oviduct Injection
Oviduct Injection - Related Videos
07:13
Alginate Hydrogels for Three-Dimensional Organ Culture of Ovaries and Oviducts
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Cited by 43 •
2011
Culture of normal cells in their three-dimensional context represents an alternative method to study early events required for cellular transformation and tumorigenesis. This method is used to grow normal ovarian and oviductal cells to study early events in ovarian cancer formation.
09:56
Improved Genome Editing via Oviductal Nucleic Acids Delivery-based In Vivo Electroporation Technique for Knockout Mice Generation
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2025
The efficiency of the Improved Genome Editing via Oviductal Nucleic Acids Delivery (I-GONAD) method is comparable to traditional microinjection, which requires zygote collection from donor females and transfer to pseudo-pregnant females. This protocol demonstrates its effectiveness by introducing CRISPR/Cas9-induced mutations into the ROSA26 locus on chromosome 6.
04:02
Embryo Transfer Into Rabbit: A Procedure for Laparoscopy-Guided Transfer of Embryos Into Oviduct of Ovulating Recipient Female Rabbit Model
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2025
This video describes the technique of transferring embryos into the oviduct of a recipient female rabbit via Laparoscopy. It allows embryo remodeling in rabbits during its migration through the oviduct, eventually helping in successful implantation. This technique can also be used for stored embryos with good efficiency.
06:12
Production of Genetically Engineered Golden Syrian Hamsters by Pronuclear Injection of the CRISPR/Cas9 Complex
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Cited by 24 •
2018
Pronuclear (PN) injection of the clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein-9 nuclease (CRISPR/Cas9) system is a highly efficient method for producing genetically engineered golden Syrian hamsters. Herein, we describe the detailed PN injection protocol for the production of gene knockout hamsters with the CRISPR/Cas9 system.
07:44
Microdissection and Dissociation of the Murine Oviduct: Individual Segment Identification and Single Cell Isolation
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Cited by 1 •
2021
A method for microdissection of the mouse oviduct that allows collection of the individual segments while maintaining RNA integrity is presented. In addition, non-enzymatic oviductal cell dissociation procedure is described. The methods are appropriate for subsequent gene and protein analysis of the functionally different oviductal segments and dissociated oviductal cells.
FAQs
Timing determines which biological target encounters the preparation. An introduction during one stage may favor contact with oviduct epithelium, whereas another timing may place the material nearer developing reproductive products. This distinction lets investigators relate observed responses to the compartment and developmental stage being examined.
Volume and composition influence how the introduced material moves through the oviduct lumen and what it can contact. They therefore help determine whether exposure is concentrated near epithelial tissues, developing reproductive products, or both. Considering these variables is essential when interpreting localized biological responses or changes associated with developing eggs.
Contact depends primarily on the interaction among injection timing, volume, and preparation composition. These factors affect movement through the lumen and the material’s access to epithelial tissues or developing reproductive products. Researchers can use that relationship to investigate oviduct function separately from effects observed in egg-associated development.
The technique directs a preparation into a defined reproductive compartment rather than treating the reproductive system as an undifferentiated whole. This focused exposure allows investigators to examine responses associated with oviduct function and to assess how introduced substances influence the composition or development of egg-associated products.
A study begins by selecting the preparation and defining the biological question, then coordinating the injection timing, volume, and composition with the intended target. The material is introduced into the oviduct lumen, after which investigators evaluate epithelial responses, reproductive products, or developing eggs according to the study objective.
Researchers may choose this approach when they need to examine gene delivery, protein production, reproductive physiology, or egg-associated development within the oviduct environment. Its value comes from connecting the introduced material with a defined reproductive compartment, enabling assessment of localized effects and changes in developing egg composition.