Pancreatic Islet Visualization
Pancreatic Islet Visualization - Related Videos
07:21
Pancreatic Islets Isolation through Collagenase Perfusion: An Enzymatic Method to Isolate Pancreatic Islet Cells
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2023
Pancreatic islets, also called the Islets of Langerhans, are a cluster of endocrine cells which produces hormones for glucose regulation and other important biological functions. This protocol explains a method for isolating pancreatic islets by first perfusing collagenase via Ampulla of Vater to digest the exocrine pancreatic tissue and a simplified density gradient centrifugation to purify the islets from mice.
03:33
Grafting Pancreatic Islets: A Method to Implant Pancreatic Islets into the Anterior Chamber of the Mouse Eye for In Vivo Imaging
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2023
This video describes the protocol for grafting human pancreatic islets into the anterior chamber of the mouse’s eye to monitor revascularization of human islet grafts in vivo. It also helps to understand the contribution of recipient versus donor cells in promoting the encapsulation and vascularization of the graft.
16:59
Computer-assisted Large-scale Visualization and Quantification of Pancreatic Islet Mass, Size Distribution and Architecture
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Cited by 14 •
2011
Novel computer-assisted methods of large-scale procurement and analysis of immunohistochemically stained pancreatic specimens are described: (1) Virtual Slice capture of the entire section; (2) Mass analysis of large-scale data; (3) Reconstruction of 2D Virtual Slices; (4) 3D islet mapping; and (5) Mathematical analysis.
14:39
A Simple and Rapid Method for Simultaneous Isolation of Primary Islets and Primary Pancreatic Acinar Cells from Mice
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2026
This study developed a simplified method to efficiently extract functional primary islets and acinar cells from the mouse pancreas, offering a valuable tool for studying intercellular communication in the pathogenesis of Acute Pancreatitis-Induced Diabetes.
09:35
A High-content In Vitro Pancreatic Islet β-cell Replication Discovery Platform
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Cited by 5 •
2016
Critical challenges for the diabetes research field are to understand the molecular mechanisms that regulate islet β-cell replication and to develop methods for stimulating β-cell regeneration. Herein a high-content screening method to identify and assess the β-cell replication-promoting activity of small molecules is presented.
FAQs
Cell identification depends on signals associated with particular endocrine populations. Fluorescent labels and genetically encoded reporters can distinguish insulin-producing beta cells from other islet cell types, while endogenous optical signals provide information without relying solely on added markers. This separation allows investigators to relate cellular identity to organization and activity within the tissue.
Time-resolved imaging adds a dynamic dimension by following changes as they occur rather than capturing structure at one moment. Measurements of calcium or hormone release can show how islet cells respond during physiological stimulation, helping researchers connect cellular activity with glucose-responsive secretion and identify altered functional behavior.
The arrangement and distribution of endocrine cells provide structural context for interpreting their behavior. Imaging can reveal how cell organization relates to function, development, or dysfunction, rather than treating each hormone-producing cell as an isolated unit. This connection is valuable when examining changes in islet organization associated with diabetes-related processes.
Fluorescent labels, genetically encoded reporters, and endogenous optical signals answer different imaging needs within the same biological system. Labels help identify cell populations, reporters support observation of selected activities, and endogenous signals can contribute optical information from the tissue itself. Combining these approaches can link cell identity, structure, and physiological behavior.
A typical workflow begins with tissue preparation, followed by selection of fluorescent labels, genetically encoded reporters, or endogenous optical signals appropriate to the question. Microscopy then captures spatial organization or time-dependent activity. Researchers analyze the resulting images to compare cell distribution, calcium behavior, hormone release, or other features relevant to islet physiology.
This approach is useful when researchers need to follow how islets form, where cell types are distributed, and how their organization changes over time or across experimental conditions. Structural imaging supplies information about architecture, while functional imaging can indicate whether developing or organized cells display appropriate activity related to endocrine function.
Imaging can connect diabetes-related cellular dysfunction with changes in islet structure, cell composition, calcium responses, or hormone release. Researchers can use these readouts to investigate disease mechanisms and assess experimental treatments by examining whether cellular organization or physiological activity changes. The resulting evidence may also inform strategies aimed at preserving or restoring endocrine function.