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Computer-assisted Large-scale Visualization and Quantification of Pancreatic Islet Mass, Size Distribution and Architecture
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Computer-assisted Large-scale Visualization and Quantification of Pancreatic Islet Mass, Size Distribution and Architecture

Computer-assisted Large-scale Visualization and Quantification of Pancreatic Islet Mass, Size Distribution and Architecture

DOI:
10.3791/2471-v

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16:59 min

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March 04, 2011

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, , , , , , , ,
1Department of Medicine,University of Chicago, 2Laboratory of Biological Modeling, National Institute of Diabetes and Digestive and Kidney Diseases,National Institutes of Health, 3Department of Surgery,University of Chicago, 4Diabetes Division,University of Massachusetts

Chapters

  • 00:05Title
  • 01:08Introduction
  • 02:00The Creation of Virtual Slices of Immunohistochemically Stained Images
  • 04:15Computer-Assisted Two-Dimensional Analysis
  • 05:42Three-Dimensional Reconstruction of Two Dimensional Immunohistochemically Stained Virtual Slice Images
  • 07:25Islet Mapping: Collecting Image Stacks
  • 10:21Islet Mapping: Mapping Image Stacks
  • 12:36Representative Results for the Large-Scale Visualization and Quantification of Pancreatic Islet Mass, Size Distribution and Architecture
  • 15:47Conclusion

Summary

Automatic Translation

Automatic Translation

Novel computer-assisted methods of large-scale procurement and analysis of immunohistochemically stained pancreatic specimens are described: (1) Virtual Slice capture of the entire section; (2) Mass analysis of large-scale data; (3) Reconstruction of 2D Virtual Slices; (4) 3D islet mapping; and (5) Mathematical analysis.

Tags

Computer-assistedLarge-scale VisualizationQuantificationPancreatic IsletIslet MassSize DistributionArchitectureEndocrine CellsBeta-cellsAlpha-cellsDelta-cellsPancreas WeightTotal Beta-cell MassIslet Size DistributionFunctional LimitLarge Animal PancreataIslet PlasticityCellular CompositionArchitectureSpecies Variation

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