Parafilm Method
Parafilm Method - Related Videos
09:33
Methods to Classify Cytoplasmic Foci as Mammalian Stress Granules
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Cited by 33 •
2017
Stress Granules (SGs) are nonmembranous cytoplasmic structures that form in cells exposed to a variety of stresses. SGs contain mRNAs, RNA-binding proteins, small ribosomal subunits, translation-related factors, and various cell signaling proteins. This protocol describes a workflow that uses several experimental approaches to detect, characterize, and quantify bona fide SGs.
03:37
An Immunofluorescence-Based Method to Quantify Replication Stress in Cancer Cells
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2025
The video demonstrates an immunofluorescence-based approach for quantifying replication stress in cancer cells. Hydroxyurea-treated cells produce single-stranded DNA, detected through immunofluorescence. The count of green fluorescence foci indicates the level of replication stress in cancer cells.
02:28
Extraction of Apoplastic Bacteria Using a Syringe-Based Pressure Method
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2025
Source: Rufián, J. S., et al, Single-Cell Analysis of the Expression of Pseudomonas syringae Genes within the Plant Tissue. J. Vis. Exp. (2022)This video demonstrates a syringe-based pressure method for extracting apoplastic bacteria, which proliferate and form microcolonies within the apoplast, the intercellular space between plant cells. The method yields debris-free bacterial samples suitable for downstream single-cell analysis.
03:23
Injection of Adult Drosophila Flies: A Method of Compound or Label Delivery
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2023
This video describes how to perform an injection of live adult flies and includes an example protocol in which labeled particles and trypan blue are injected in fly abdomens to assay phagocytosis.
01:29
Methods of Sterilization I: Physical Methods
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2025
As used in a healthcare facility, sterilization destroys all microorganisms through physical or chemical methods. The physical method includes steam, dry heat, boiling water, and radiation. Steam sterilization uses non-toxic, low-cost moist heat in the form of saturated steam under pressure, which is fast, microbicidal, and sporicidal, and quickly warms and penetrates fabrics. Autoclaves, or steam sterilizers, expose each item to direct steam contact for a predetermined time at the necessary...
FAQs
Stretching the film allows it to conform closely to the rim of a tube, flask, plate, or similar container. Its flexibility helps maintain contact around the opening during routine handling, creating a temporary barrier rather than a permanent closure. This fit helps reduce opportunities for airborne particles or moisture to enter or escape.
The method helps limit contamination, moisture loss, and exposure to airborne particles. These effects are useful when biological contents remain in a container during handling, incubation, transport, or storage. The film should be understood as a practical supplement to standard laboratory closures, not as an unlimited substitute for them.
Flexibility allows the film to adapt to different vessel openings and maintain a close fit as the container is handled. Unlike a rigid covering, it can be stretched around the rim and removed when access is needed. This combination of adaptability and removability makes the method convenient for temporary protection during experiments.
To apply the method, place the film over or around the opening of the selected container, stretch it around the vessel rim, and press it into conformity with that edge. After the experiment or handling period, remove the film when access is required. The process is quick and requires no complex equipment.
Biologists may use the method when containers holding cell cultures, microbial cultures, or biological samples need temporary protection. It can support work during incubation, transport, or storage by helping limit exposure and moisture loss. Its low cost, quick application, and easy removal are especially useful for routine laboratory workflows.
For cell and microbial cultures, the film provides an added protective barrier around the container opening during incubation or routine handling. It can help limit airborne exposure and moisture loss while the culture remains in its vessel. Because it supplements standard closures, researchers can use it as an additional practical measure rather than the sole closure system.