Rat Liver Tissue
Rat Liver Tissue - Related Videos
11:08
The Use of Gas Chromatography to Analyze Compositional Changes of Fatty Acids in Rat Liver Tissue during Pregnancy
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Cited by 71 •
2014
Pregnancy leads to significant changes to the fatty acid composition of maternal tissues. Lipid profiles can be obtained via gas chromatography to allow identification and quantification of fatty acids in individual lipid classes among rats fed various high and low fat diets during pregnancy.
09:27
The Dimethylnitrosamine Induced Liver Fibrosis Model in the Rat
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Cited by 11 •
2016
We describe a method to produce an animal model of liver fibrosis in the rat, and assess the degree of fibrosis by histological examination of the liver. The model can be used to study the development of liver disease as well as to test the efficacy of potential anti-fibrotic agents.
07:39
Isolation of Rat Portal Fibroblasts by In situ Liver Perfusion
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Cited by 22 •
2012
A technique for isolating portal fibroblasts from rat liver is described. Livers are perfused and digested in situ with collagenase, followed by ex vivo digestion of the liver slurry and size selection of cells. This method provides a pure population of portal fibroblasts without the need for passage in culture.
18:25
Orthotopic Liver Transplantation in Rats
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Cited by 31 •
2012
We present an easy-to-establish revision of the classical two-cuff technique for orthotopic liver transplantation in rat.
09:38
Procedure for Decellularization of Rat Livers in an Oscillating-pressure Perfusion Device
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Cited by 18 •
2015
The presented techniques for liver harvesting, cannulation and perfusion using our proprietary device enable sophisticated perfusion set-ups to improve decellularization and recellularization experiments in rat livers.
FAQs
The portal vein delivers nutrient-rich blood, while the hepatic artery supplies oxygenated blood. Their separate contributions expose hepatocytes and other lobular structures to substrates and oxygen needed for metabolic activity. This organized circulation helps researchers examine how nutrient availability, oxygenation, and blood-borne substances affect liver physiology, chemical processing, and responses to injury.
Hepatocytes use enzyme-mediated reactions to modify drugs and toxins. These reactions make rat liver tissue useful for investigating how chemicals are processed and how exposure may alter cellular responses. Toxicology studies can therefore connect a tested substance with changes in liver function, helping researchers examine potential injury mechanisms or treatment effects.
Hepatocytes release bile into canaliculi, specialized channels associated with the tissue's lobular organization. This secretory route provides a way to study how liver cells coordinate production and movement of bile-related material. Examining this function can help researchers investigate cellular responses to altered conditions, injury, or treatments that affect secretory activity.
Fresh, preserved, and cultured samples provide different ways to examine rat liver tissue. Fresh material can support analysis of current tissue responses, preserved samples allow structural or cellular features to be examined after collection, and cultured samples provide a setting for observing responses to chemicals, nutritional changes, injury, or potential treatments.
Researchers can use the tissue to examine cellular responses to chemical exposure, nutritional changes, injury, and potential therapeutic treatments. These experimental conditions connect external influences with liver physiology and metabolism. Comparing responses across conditions can help clarify how hepatocytes and associated tissue structures react during toxicology, disease-related studies, or treatment evaluation.
Its organized combination of hepatocytes, vascular cells, connective tissue, and bile-duct structures provides several tissue contexts for examining liver responses. Researchers can study how injury affects these components, how the tissue responds during regeneration, and how disease-related changes influence metabolism, detoxification, or secretion. This makes the model relevant to both basic biology and therapeutic research.