Reverse Transcription
Reverse Transcription - Related Videos
04:08
Real-Time Quantitative Reverse Transcription PCR for Diagnosing Viral Infections
0 Views •
2025
This video demonstrates a quantitative method for detecting viral infection using real-time reverse transcription PCR. The reverse transcription step converts the RNA to cDNA, which is then amplified via PCR cycles. The presence of viral RNA in the sample is confirmed by analyzing the amplification and dissociation curves of DNA obtained from the PCR.
02:27
Measuring Hepatitis C Viral Load Using Quantitative Reverse Transcription Polymerase Chain Reaction
0 Views •
2026
Source: Ren, S., et al. A Protocol for Analyzing Hepatitis C Virus Replication. J. Vis. Exp. (2014).This video demonstrates the procedure for quantifying Hepatitis C viral RNA using quantitative reverse transcription PCR to measure viral load in infected cell cultures.
07:18
High Throughput In Vitro Assessment of Latency Reversing Agents on HIV Transcription and Splicing
0 Views •
2019
A high throughput protocol for functional assessment of HIV efficient reactivation and clearance of latent proviruses is described and applied by testing the impact of interventions on HIV transcription and splicing. Representative results of the effect of latency reversing agents on LTR-driven transcription and splicing are provided.
10:44
Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp
0 Views •
Cited by 15 •
2018
This protocol describes the critical steps and precautions required to perform single cell multiplex reverse transcription polymerase chain reaction after patch-clamp. This technique is a simple and effective method to analyze the expression profile of a predetermined set of genes from a single cell characterized by patch-clamp recordings.
12:12
Analysis of Termination of Transcription Using BrUTP-strand-specific Transcription Run-on (TRO) Approach
0 Views •
Cited by 10 •
2017
We describe a basic experimental approach for analysis of termination of transcription by RNA polymerase II in vivo using BrUTP by the strand-specific transcription run-on (TRO) approach in budding yeast. This protocol can be extended to study transcription termination by other RNA polymerases both in yeast and higher eukaryotes.
FAQs
Primer availability determines where synthesis can begin: reverse transcriptase does not initiate the new strand without a primer. After binding the RNA template, the enzyme extends from that starting point by adding complementary DNA nucleotides. This ordered sequence of initiation and extension converts transcript information into cDNA that can be retained for subsequent analysis.
Retroviruses use reverse transcription as part of their replication strategy, making the enzyme relevant to both molecular biology and retroviral biology. In this context, RNA supplies the starting information for a DNA copy. The same reaction is therefore studied in laboratories and in biology to understand retroviral replication.
The cDNA product is important because it preserves information originally present in an RNA molecule in a more stable DNA form. That preserved copy can then support downstream work such as RT-PCR, cDNA library construction, and gene-expression analysis, allowing researchers to investigate transcript information after the reverse-transcription step.
RT-PCR combines reverse transcription with polymerase chain reaction to connect RNA measurement with DNA-based analysis. The first stage creates cDNA from the RNA sample; the subsequent PCR-based workflow uses that copied information to assess transcript quantities. This makes the method useful for examining gene expression rather than merely producing a DNA copy.
A cDNA library converts information from RNA molecules into a set of stable DNA copies that can be examined as a collection. This is useful when researchers want to preserve and investigate transcript information rather than work only with the original RNA molecules. The approach supports broader studies of RNA-derived information.
Researchers apply the resulting cDNA-based workflows to gene-expression analysis and to questions about cellular responses, development, and disease. By converting RNA transcript information into a form suitable for analysis, reverse transcription helps connect changes in RNA abundance with biological states. Its value therefore spans basic biology and disease-focused investigation.