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JoVE Journal
Immunology and Infection

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High Throughput In Vitro Assessment of Latency Reversing Agents on HIV Transcription and Splicing
 

High Throughput In Vitro Assessment of Latency Reversing Agents on HIV Transcription and Splicing

Article DOI: 10.3791/58753-v
January 22nd, 2019

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Summary January 22nd, 2019

A high throughput protocol for functional assessment of HIV efficient reactivation and clearance of latent proviruses is described and applied by testing the impact of interventions on HIV transcription and splicing. Representative results of the effect of latency reversing agents on LTR-driven transcription and splicing are provided.

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