Spirochete Habitat

Spirochete habitat refers to the natural environments where these slender, spiral-shaped bacteria survive, grow, and interact with hosts or surrounding microbial communities. Their flexible cells and internal axial filaments generate distinctive corkscrew motility, allowing them to move through viscous materials such as mucus, connective tissue, sediment, and water. Spirochetes occupy diverse niches, including freshwater and marine environments, soils, animal microbiomes, and human tissues, where some species exist harmlessly while others cause disease. Studying their habitats helps explain transmission, host colonization, ecological adaptation, and the environmental conditions that influence infections and microbial diversity.

Spirochete Habitat - Related Videos

Education

JoVE Core - Biology

Habitat Fragmentation

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2020

Habitat fragmentation describes the division of a more extensive, continuous habitat into smaller, discontinuous areas. Human activities such as land conversion, as well as slower geological processes leading to changes in the physical environment, are the two leading causes of habitat fragmentation. The fragmentation process typically follows the same steps: perforation, dissection, fragmentation, shrinkage, and attrition. Perforation and dissection often occur during the initial stages of...

Research

JoVE EoE - Bacterial Pathogenesis and Host Interactions

Cultivation of Pathogenic Spirochetes to Investigate Skin Infection

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2026

Source: Berthold, A., et al. Cultivation Methods of Spirochetes from Borrelia burgdorferi Sensu Lato Complex and Relapsing Fever Borrelia. J. Vis. Exp.(2022)This video demonstrates the cultivation of pathogenic spirochetes from infected skin tissue to model dermal infection. It highlights their localization in the dermis, interaction with the extracellular matrix, and survival during selective culture conditions.

Bacteriophage-Mediated Gene Delivery in Spirochete Bacteria

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2026

Source: Eggers, C. H. Phage-Mediated Genetic Manipulation of the Lyme Disease Spirochete Borrelia burgdorferi. J. Vis. Exp. (2022).This video demonstrates the transduction of genetically modified Borrelia spirochetes using polyethylene glycol–precipitated, non-infectious bacteriophages carrying a kanamycin-resistance plasmid. After incubation with the bacteria, the culture is plated on antibiotic-supplemented medium. Only cells that receive the plasmid survive and form colonies. These colonies...

Methods for Characterizing the Co-development of Biofilm and Habitat Heterogeneity

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Cited by 5 •

2015

Biofilms have complex interactions with their surrounding environment. To comprehensively investigate biofilm-environment interactions, we present here a series of methods to create heterogeneous chemical environment for biofilm development, to quantify local flow velocity, and to analyze mass transport in and around biofilm colonies.

Research

JoVE Journal - Immunology and Infection
Free Sample

Procedures for In Vitro Cultivation of Treponema pallidum, the Syphilis Spirochete

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Cited by 1 •

2025

This protocol describes in vitro cultivation of the syphilis pathogen Treponema pallidum subsp. pallidum in co-culture with mammalian cells. The method is scalable; it can be used to produce large quantities of T. pallidum and to generate clonal cultures.

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