Lactic Acid Bacteria
Lactic Acid Bacteria - Related Videos
03:24
Revival and Growth of Lactic Acid Bacteria
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2025
Source: Ayivi, R. et al., The Cultivation, Growth, and Viability of Lactic Acid Bacteria: A Quality Control Perspective. J. Vis. Exp. (2022)This video demonstrates the method for recovering cold-stressed lactic acid bacteria from a frozen glycerol stock, restoring bacterial viability through anaerobic incubation, and isolating active colonies for culture expansion.
04:40
The Cultivation, Growth, and Viability of Lactic Acid Bacteria: A Quality Control Perspective
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Cited by 6 •
2022
The quality control assessment of lactic acid bacteria (LAB) cultures has been confirmed as an effective way to enhance the viability and functionality of LAB strains for fermentation procedures. To buttress this assertion, we developed a protocol that elucidates how LAB cultures are activated and cultivated for fermentation and bioprocessing procedures.
10:23
Characterizing Mediated Extracellular Electron Transfer in Lactic Acid Bacteria with a Three-Electrode, Two-Chamber Bioelectrochemical System
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Cited by 4 •
2024
Here we present a protocol for characterizing mediated extracellular electron transfer (EET) in lactic acid bacteria using a three-electrode, two-chamber bioelectrochemical system. We illustrate this method with Lactiplantibacillus plantarum and the redox mediator 1,4-dihydroxy-2-naphthoic acid and provide a thorough description of the electrochemical techniques used to evaluate mediated EET.
11:28
High-Throughput Screening of L-Lactic Acid-Hyperproducing Bacillus coagulans Mutants Using an Automated Droplet Microfluidic Platform
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2026
This protocol presents a standard high-throughput screening workflow for industrial microorganisms. It involves constructing a mutant library via ARTP mutagenesis, performing positive droplet screening with automated microfluidics, and subsequently verifying the results. The workflow’s reliability was validated by screening high-yield B. coagulans strains.
09:31
Sustained Administration of β-cell Mitogens to Intact Mouse Islets Ex Vivo Using Biodegradable Poly(lactic-co-glycolic acid) Microspheres
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Cited by 2 •
2016
Here, we present methodology to generate and administer compound of interest-loaded poly(lactic-co-glycolic acid) (PLGA) microspheres to intact mouse islets in culture with subsequent immunofluorescence analysis of β-cell proliferation. This method is suitable for determining the efficacy of candidate β-cell mitogens.
FAQs
During carbohydrate fermentation, glycolysis and related fermentation pathways process carbohydrate-derived sugars and convert them into lactic acid. This provides the microorganisms with energy while producing an acidic end product. Linking energy acquisition to acid formation explains why their metabolism can simultaneously sustain bacterial activity and alter the surrounding chemical environment.
The accumulation of lactic acid lowers environmental pH, making conditions less favorable for competing microorganisms. This is not simply a chemical by-product; it links bacterial metabolism to ecological change. In a food or microbial community, the resulting acidity can help determine which organisms persist, making pH reduction central to fermentation outcomes and microbial competition.
Their biological effects extend beyond individual cells. Carbohydrate use and acid production can influence interactions within microbial communities by changing the conditions experienced by neighboring organisms. This makes lactic acid bacteria useful for studying microbial ecology, because researchers can examine how metabolism modifies a shared environment and how those changes shape community relationships.
Food production takes advantage of their metabolic activity in yogurt, cheese, fermented vegetables, and other foods. By converting available sugars into lactic acid, these microorganisms contribute to the characteristic outcomes of fermentation. Their activity can affect preservation, flavor, and texture, so they influence both the stability and sensory properties of fermented products.
Lactic acid bacteria are relevant in both natural and engineered systems because fermentation can be examined as a biological process outside food production. In natural settings, their activity informs studies of microbial ecology. In engineered settings, it helps researchers investigate how metabolism can alter environmental conditions, connecting fundamental biology with applied fermentation research.
Studying lactic acid bacteria connects cellular metabolism with larger-scale biological effects. Their fermentation pathways illustrate how microorganisms obtain energy, while their acid production shows how one metabolic activity can influence competitors, communities, and food systems. This makes them useful for research spanning microbial physiology, ecology, food biology, and engineered biological systems.