Subretinal Space Access
Subretinal Space Access - Related Videos
07:13
An Alternative and Validated Injection Method for Accessing the Subretinal Space via a Transcleral Posterior Approach
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Cited by 19 •
2016
Subretinal injections are the most common technique for delivering large therapeutic agents such as proteins and viral vectors to photoreceptors and the retinal pigment epithelium. An alternative method in mice that successfully targets the subretinal space with minimal collateral damage and fast recovery times is described here.
05:09
Delivery of a Fluorescent Dye into the Subretinal Space of a Mouse
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2025
Source: Parikh, S., et al. An Alternative and Validated Injection Method for Accessing the Subretinal Space via a Transcleral Posterior Approach. J. Vis. Exp. (2016)This video demonstrates the procedure for delivering a fluorescent dye into the subretinal space of a mouse. The process involves anesthetizing the mouse, preparing the eye, making precise incisions to access the subretinal space, and injecting the dye to create a controlled retinal detachment. This technique allows for targeted...
07:54
Accessing the Subdural Space of the Rodent Spinal Cord for Treatment Delivery
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2025
This surgical protocol is a step-by-step guide to insert an intrathecal catheter into the spinal subdural space of a rodent to deliver targeted treatment.
06:04
Performing Subretinal Injections in Rodents to Deliver Retinal Pigment Epithelium Cells in Suspension
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Cited by 32 •
2015
Here we present a community accepted protocol in multimedia format for subretinally injecting a bolus of RPE cells in rats and mice. This approach can be used for determining rescue potentials, safety profiles, and survival capacities of grafted RPE cells upon implantation in animal models of retinal degeneration.
05:09
Subretinal Injection of Gene Therapy Vectors and Stem Cells in the Perinatal Mouse Eye
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Cited by 35 •
2012
This surgical technique illustrates the injection of gene therapy vectors and stem cells into the subretinal space of the mouse eye.
FAQs
The technique uses a fine cannula to pass through a carefully created retinal opening, followed by controlled infusion beneath the neurosensory retina. Fluid can form a localized bleb that separates the retina from the retinal pigment epithelium. This controlled separation creates temporary access for placing or manipulating materials near the intended target tissue.
Positioning materials beneath the retina places them close to photoreceptors and retinal pigment epithelial cells, the tissues specifically identified as targets for localized intervention. This proximity can support more focused delivery than exposing surrounding ocular structures. The approach is therefore relevant when a study or therapy requires contact with these retinal layers rather than broad distribution within the eye.
A controlled retinal opening and careful infusion are central to maintaining the intended subretinal location. The cannula must enter through the retina, while the infused material creates or supports a localized bleb beneath it. These steps determine whether fluid, cells, or therapeutic agents remain positioned near the target layers instead of spreading unnecessarily to surrounding ocular structures.
Subretinal placement is designed to localize an intervention between the neurosensory retina and retinal pigment epithelium. By directing material into this potential space, the approach can limit exposure of surrounding ocular structures compared with less targeted placement. Its value lies in combining access to specific retinal layers with a delivery pattern intended to remain localized.
A typical sequence begins by creating a controlled retinal opening, followed by introducing a fine cannula through that opening. The operator then carefully infuses the selected material beneath the retina, often producing a localized bleb. Depending on the study or intervention, the infused substance may be fluid, cells, or a therapeutic agent intended for targeted retinal delivery.
The approach can accommodate several classes of material identified in the source context: fluid, cells, and therapeutic agents. Fluid may be used to create the subretinal bleb, whereas cells or therapeutic agents can be placed near photoreceptors and retinal pigment epithelial cells. The selected material depends on whether the goal is access, manipulation, or treatment development.
Subretinal Space Access supports investigations and treatment development for inherited retinal disorders, geographic atrophy, and other conditions requiring localized intervention. Researchers can use the approach to position cells or therapeutic agents near relevant retinal layers. Its application is especially suited to studies where proximity to photoreceptors or retinal pigment epithelial cells is important for evaluating a targeted intervention.