Surrogate Mother

A surrogate mother is a woman who carries a pregnancy and gives birth for intended parent or parents, making surrogacy an important reproductive biology and assisted reproduction topic. In gestational surrogacy, clinicians create an embryo through in vitro fertilization and transfer it into the surrogate’s uterus after preparing the uterine lining, so she typically has no genetic relationship to the child; traditional surrogacy uses the surrogate’s own egg. Surrogacy can help people affected by infertility, medical conditions, or absent reproductive organs pursue parenthood. Its study also connects implantation, placental development, pregnancy physiology, genetic relationships, and the medical and ethical considerations of reproductive care.

Surrogate Mother - Related Videos

Research

JoVE Journal - Bioengineering

Preparation and Analysis of In Vitro Three Dimensional Breast Carcinoma Surrogates

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Cited by 6 •

2016

We demonstrate a method to generate 3D breast cancer surrogates, which can be cultured using a perfusion bioreactor system to deliver oxygen and nutrients. Following growth, surrogates are fixed and processed to paraffin for evaluation of parameters of interest. The evaluation of one such parameter, cell density, is explained.

Microglia as a Surrogate Biosensor to Determine Nanoparticle Neurotoxicity

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Cited by 16 •

2016

Microglia (immune cells of the brain), are used as a surrogate biosensor to determine how nanoparticles influence neurotoxicity. We describe a series of experiments designed to assay microglial response to nanoparticles and exposure of hypothalamic neurons to supernatant from activated microglia to determine neurotoxicity.

Research

JoVE Journal - Engineering
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Surrogate Model Development for Digital Experiments in Welding

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Cited by 2 •

2025

This protocol outlines a general workflow for constructing an artificial neural network for welding simulation using datasets generated automatically through Python scripts with macro functions. The workflow is validated against a benchmark case involving single-pass, straight-line welding. The surrogate model's predictive accuracy shows a strong agreement with finite element simulations.

Measuring Relative Insulin Secretion using a Co-Secreted Luciferase Surrogate

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Cited by 7 •

2019

This protocol describes how to perform rapid low-cost luciferase assays at medium-throughput using an insulin-linked Gaussia luciferase as a proxy for insulin secretion from beta cells. The assay can be performed with most luminescence plate readers and multichannel pipettes.

Determining Nanoparticle-Induced Toxicity in Neurons Using Microglia as a Surrogate Biosensor

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2025

This video demonstrates a method to assess the neurotoxic effects of nanoparticles using microglia. It outlines the steps for activating microglia with nanoparticles, filtering conditioned media to isolate microglia-derived pro-inflammatory cytokines, and testing hypothalamic neuron toxicity with the conditioned media.

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