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Measuring Relative Insulin Secretion using a Co-Secreted Luciferase Surrogate
 

Measuring Relative Insulin Secretion using a Co-Secreted Luciferase Surrogate

Article DOI: 10.3791/59926-v
June 25th, 2019

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Summary June 25th, 2019

This protocol describes how to perform rapid low-cost luciferase assays at medium-throughput using an insulin-linked Gaussia luciferase as a proxy for insulin secretion from beta cells. The assay can be performed with most luminescence plate readers and multichannel pipettes.

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