Synechocystis Pcc 6803
Synechocystis Pcc 6803 - Related Videos
08:07
Spectrophotometric Determination of Phycobiliprotein Content in Cyanobacterium Synechocystis
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Cited by 42 •
2018
Here, we present a protocol to quantitatively determine phycobiliprotein content in the cyanobacterium Synechocystis using a spectrophotometric method. The extraction procedure was also successfully applied to other cyanobacteria and algae strains; however, due to variations in pigment absorption spectra, it is necessary to test the spectrophotometric equations for each strain individually.
03:54
Extraction and Purification of Plastoglobules from Photosynthetic Bacteria
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2025
Source: Shivaiah, K., et al. Plastoglobule Lipid Droplet Isolation from Plant Leaf Tissue and Cyanobacteria. J. Vis. Exp. (2022)The video demonstrates the isolation of plastoglobules from cyanobacteria. It begins with a culture of cyanobacteria and proceeds through cell disruption using mechanical force. The release of photosynthetic pigments indicates successful lysis. A sucrose step gradient is then used to separate cellular components by density, allowing low-density plastoglobules to form a...
08:43
Application of Granger Causality Analysis of the Directed Functional Connection in Alzheimer's Disease and Mild Cognitive Impairment
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Cited by 7 •
2017
Based on resting-state functional magnetic resonance imaging with Granger causality analysis, we investigated the alterations in the directed functional connectivity between the posterior cingulate cortex and whole brain in patients with Alzheimer's Disease (AD), patients with Mild Cognitive Impairment (MCI), and healthy controls.
02:16
Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes
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2020
The present-day mitochondrial and chloroplast genomes have retained some of the characteristics of their ancestral prokaryotes and also have acquired new attributes during their evolution within eukaryotic cells. Like prokaryotic genomes, mitochondrial and chloroplast genomes neither bind with histone-like proteins nor show complex packaging into chromosome-like structures, as observed in eukaryotes. Unlike mitotic cell divisions observed in eukaryotic cells, mitochondria and chloroplasts...
11:45
Generation of Marked and Markerless Mutants in Model Cyanobacterial Species
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Cited by 36 •
2016
Introducing multiple genomic alterations into cyanobacteria is an essential tool in the development of strains for industrial and basic research purposes. We describe a system for generating unmarked mutants in the model cyanobacterial species Synechocystis sp. PCC6803 and marked mutants in Synechococcus sp. PCC7002.
FAQs
Natural competence allows Synechocystis sp. PCC 6803 to take up externally supplied DNA, while homologous recombination supports its integration into corresponding genomic regions. Together, these properties provide a route for altering selected genes rather than relying only on spontaneous variation. Researchers can then compare modified and parental strains to connect genetic changes with biological phenotypes.
Targeted mutations help researchers test the function of individual genes by observing how their alteration changes cellular behavior. In this organism, that strategy can clarify contributions to oxygenic photosynthesis, carbon fixation, circadian regulation, or stress acclimation. Linking a defined genetic change to a measurable phenotype strengthens mechanistic interpretations of biological processes.
Controlled light, carbon, and nutrient conditions allow researchers to examine how environmental inputs shape cellular responses. Changing one of these conditions can reveal effects on photosynthetic activity, carbon-related processes, or acclimation to stress. Maintaining defined conditions also makes comparisons among strains more informative, particularly when evaluating mutations or altered gene expression.
A typical investigation introduces a targeted genetic change or modifies gene expression, grows the resulting strain under defined environmental conditions, and examines its phenotype. Researchers may compare the modified strain with an unmodified reference under different light, carbon, or nutrient regimes. This workflow connects genotype, environmental context, and observable biological response.
Studies with this organism can examine photosynthetic electron transport, carbon fixation, circadian regulation, and responses to environmental stress. These areas span energy conversion, carbon utilization, biological timing, and acclimation. Because the genome is sequenced and genetic changes are tractable, researchers can investigate how specific genes or expression patterns contribute to these processes.
Engineered strains provide platforms for studying the production of biofuels and other biochemicals. Genetic modification and regulated gene expression allow researchers to investigate how cellular functions can be redirected toward useful products. This application connects basic work on photosynthesis, carbon fixation, and microbial genetics with broader efforts to explore sustainable biological production systems.