Drg Dissection
Drg Dissection - Related Videos
09:14
DiI-Labeling of DRG Neurons to Study Axonal Branching in a Whole Mount Preparation of Mouse Embryonic Spinal Cord
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Cited by 20 •
2011
The stereotyped projections of sensory afferents into the rodent spinal cord offer an easily accessible experimental system to study axonal branching through the tracing of single axons.
03:44
Co-Culture of Schwann Cells with DRG Neurons on a Pre-Stretched Membrane
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2025
The video demonstrates the co-culturing of dorsal root ganglion (DRG) neurons with Schwann cells. DRG neurons are first grown on a pre-stretched membrane for aligned axon growth. Schwann cells are then introduced, which attach to the axons, insulating them and promoting neural cell survival and function.
04:25
Isolation of DRG Neurons and Coculture with Schwann Cell Precursors to Generate Schwann Cells
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2025
This video demonstrates a procedure for isolating dorsal root ganglion (DRG) neurons from rat embryos and co-culturing them with Schwann cell precursors to generate mature Schwann cells. The isolated DRGs are processed to isolate neurons and glial cells. The isolated cells are cultured to purify the neurons, which are then co-cultured with Schwann cell-like cells (SCLCs) in a co-culture medium. Interaction with the DRG neurons leads to the maturation of SCLCs into Schwann cells.
02:49
Harvesting DRG from Rat Spine: A Procedure to Extract Dorsal Root Ganglion from the Spine of a Rat Model
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2023
In this video, we demonstrate the extraction of dorsal root ganglion or DRG from a harvested rat spine for downstream applications.
10:18
Dissection of Organs from the Adult Zebrafish
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Cited by 168 •
2010
This protocol describes a procedure for identifying and dissecting organs from the adult zebrafish.
FAQs
They provide access to sensory neuron cell bodies that connect peripheral tissues with the spinal cord. This makes it possible to examine how tumors, metastatic growth, or anticancer treatments affect sensory neurons directly. Findings can help connect changes in peripheral neural signaling with the development of pain during cancer progression or treatment.
The experimental system is designed to reveal whether tumor-related conditions or therapeutic exposure alter sensory neurons. Researchers can then relate those neuronal effects to cancer-associated pain mechanisms. Examining these responses separately from broader tumor biology helps clarify how metastatic growth and treatment effects may influence communication between peripheral tissues and the spinal cord.
After isolation, DRGs can support culture, imaging, electrophysiology, or molecular analysis. These approaches provide complementary views of sensory neurons, including their observable properties, electrical behavior, and molecular features. Using more than one analysis can help investigators connect cellular changes with neural signaling and evaluate their relevance to cancer-associated pain.
The procedure begins by exposing the spinal column and removing the vertebral tissue needed to access the ganglia. The DRGs are then located beside the spinal cord and carefully separated. The isolated tissue can subsequently be prepared for culture, imaging, electrophysiology, or molecular analysis, depending on the research question.
Careful separation preserves the isolated ganglia as a usable experimental preparation for downstream analysis. Because the procedure requires locating the ganglia beside the spinal cord and removing them after vertebral exposure, precise handling is central to obtaining tissue suitable for culture, imaging, electrophysiology, or molecular measurements in cancer studies.
Investigators can use dissected DRGs to evaluate how tumors, metastatic growth, or anticancer treatments affect sensory neurons. The resulting observations support analysis of peripheral neural signaling, investigation of cancer-associated pain mechanisms, and assessment of potential therapeutic interventions. Thus, the preparation links neural responses with tumor biology in a focused experimental setting.