Murine Model Crypts
Murine Model Crypts - Related Videos
04:37
Intestinal Crypts Isolation: A Method to Isolate Whole Crypts from Small Intestine of Murine Model
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2023
In this video, we demonstrate a technique to isolate whole crypts from a mouse’s small intestine. The isolated whole crypts can be used to propagate long-lived and self-renewing 3D organoids in vitro.
06:57
Generation of Murine Primary Colon Epithelial Monolayers from Intestinal Crypts
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Cited by 12 •
2021
In this protocol, we describe how to generate murine primary epithelial colon monolayers directly from intestinal crypts. We provide experimental approaches to generate confluent monolayers on permeable filters, confluent monolayers for scratch wound healing and biochemical studies, and sparse and confluent monolayers for immunofluorescence analysis.
04:35
Murine Ileal Resection Model: Studying Ileectomy-Induced Bile Metabolism in Murine Model
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2023
This video describes the detailed protocol for intestinal resection in a murine model. This technique provides insights into ileectomy-induced bile malabsorption, overaccumulation, and toxicity in the mouse intestine.
08:39
Murine Colitis Modeling using Dextran Sulfate Sodium (DSS)
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Cited by 113 •
2010
Dextran sulfate sodium (DSS) administered in the drinking water is an established murine inflammatory injury model of acute colitis. This protocol outlines the method for DSS treatment and the preparation of tissues.
03:47
A Murine Model of Carotid Aneurysm Formation
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2025
The present protocol describes a standardized surgical method for the induction of carotid aneurysms in a murine model through a single surgical operation.
FAQs
These niche signals create the conditions that allow stem cells within isolated crypts to remain active and produce three-dimensional intestinal organoids. Wnt supports the stem-cell-associated program, while R-spondin and Noggin contribute to the signaling environment needed for epithelial growth and organization. Adjusting this combination helps investigators maintain cultures suitable for studying renewal and disease-related changes.
The gel provides a three-dimensional environment in which crypt-derived cells can organize into organoids rather than growing only as a simplified surface layer. This setting supports development of key intestinal epithelial cell types and preserves structural features relevant to tissue renewal. Consequently, researchers can examine epithelial behavior in a controlled culture system while manipulating defined niche signals.
Parallel cultures allow investigators to distinguish ordinary epithelial renewal from changes associated with oncogenic transformation. By examining how normal and transformed crypt-derived organoids grow and organize under comparable conditions, researchers can analyze mutation-linked effects on tissue behavior and identify features associated with tumor initiation. These comparisons provide a controlled bridge between normal biology and cancer-related epithelial changes.
The workflow begins with isolating intestinal crypts from mouse tissue, placing them in an extracellular-matrix gel, and supplying niche signals including Wnt, R-spondin, and Noggin. Under these culture conditions, crypt-associated stem cells generate three-dimensional organoids containing key epithelial cell types. The resulting cultures can then be examined for regeneration, oncogenic effects, or treatment responses.
Researchers can expose crypt-derived organoids to candidate treatments after establishing cultures under controlled signaling conditions. Responses can be evaluated in relation to epithelial growth, regeneration, or differences between normal and transformed tissue. This approach supports early comparison of treatment effects in a defined ex vivo setting before findings are integrated with evidence from whole-animal studies.
Ex vivo crypt cultures isolate epithelial and niche-related variables that are difficult to examine separately in an intact animal. Investigators can connect specific oncogenic mutations or treatment conditions with organoid-level changes while maintaining a three-dimensional tissue context. Whole-animal studies remain relevant, but the culture system adds a tractable intermediate model for interpreting regeneration, tumor initiation, and therapy-related observations.