Peritoneal Lavage Fluid
Peritoneal Lavage Fluid - Related Videos
04:59
Peritoneal Low-density Neutrophil Isolation: A Technique to Obtain Low-density Neutrophils from Peritoneal Lavage Fluid Using Magnetic Activated Cell Sorting
0 Views •
2023
In this video, we describe the procedure for isolating low-density neutrophils, or LDNs, from the peritoneal lavage fluid of a postoperative human patient. The LDNs thus obtained can be used in further downstream experiments to understand their role in tumor growth and progression.
03:37
Visualization of Extracellular Traps in Macrophages from Human Bronchoalveolar Lavage Fluids
0 Views •
2025
This video demonstrates the immunolabeling of macrophage extracellular traps, METs, in bronchoalveolar macrophages from bronchoalveolar lavage fluid, and their visualization using a confocal microscope.
02:17
Bronchoalveolar Lavage Fluid Collection from a Mouse Model Infected with Non-typeable Haemophilus influenzae
0 Views •
2025
Source: Venuprasad, K., et al. Intra-tracheal Administration of Haemophilus influenzae in Mouse Models to Study Airway Inflammation. J. Vis. Exp. (2016)This video demonstrates the collection and processing of bronchoalveolar lavage fluid from a euthanized mouse infected with non-typeable Haemophilus influenzae for downstream analysis.
03:03
Identification and Enumeration of Immune Cells in Bronchoalveolar Lavage Fluid by Flow Cytometry
0 Views •
2025
This video demonstrates the use of flow cytometry to identify and count various immune cells in bronchoalveolar lavage fluid. Initially, single immune cells are identified using a viability dye, followed by differential staining of surface marker proteins, enabling the precise enumeration of each cell type.
09:58
A Reversible, Non-invasive Method for Airway Resistance Measurements and Bronchoalveolar Lavage Fluid Sampling in Mice
0 Views •
Cited by 10 •
2010
Repeated measurements of rodent respiratory physiology and sampling of airway inflammatory cells are desirable, but generally not feasible. Here we describe a repeatable method for orally intubating mice that permits repeated measurements of airway hyperreactivity and sampling of airway inflammatory cells.
FAQs
The recovered sample can reveal microscopic tumor involvement because washing mobilizes cells and soluble materials present on the abdominal surface. Malignant cells may therefore be detected even when disease is not readily characterized by visible findings alone. This makes lavage analysis useful for investigating limited peritoneal dissemination and refining research assessments of abdominal cancer spread.
Different analytical approaches answer different questions. Cytological examination focuses on whether malignant cells are present, while cell analysis can characterize the recovered cellular population. Molecular testing examines cancer-associated molecular signals in the fluid, including biomarkers. Using these readouts separately or together allows investigators to study cellular evidence and soluble or molecular evidence of disease.
Peritoneal lavage fluid is especially relevant when researchers evaluate peritoneal dissemination, meaning spread of cancer across the abdominal lining. Findings from the recovered fluid can add evidence about microscopic involvement that may not be captured by a tissue biopsy from one site or by imaging. It therefore supports broader investigation of disease extent and stage.
Analysis of malignant cells and cancer-associated biomarkers in the recovered fluid can contribute to treatment-response studies. These measurements provide a fluid-based research readout that complements tissue biopsy and imaging, allowing investigators to examine whether evidence of peritoneal tumor involvement changes in relation to therapy. The approach is particularly relevant when studying abdominal cancers with possible peritoneal spread.
Collection requires introducing sterile saline or another lavage solution into the peritoneal cavity, circulating it across the abdominal surface, and withdrawing the fluid for analysis. The recovered material can then undergo cytological examination, cell analysis, or molecular testing. These workflows help investigators assess malignant cells and cancer-associated biomarkers in the abdominal sample.
Its research value comes from integrating lavage findings with other assessments rather than interpreting the sample in isolation. Investigators can combine cytological, cellular, and molecular results with biopsy and imaging to study microscopic tumor spread, peritoneal dissemination, disease stage, and treatment response. This combined approach supports investigations of abdominal cancers involving the peritoneal surface.