Tracheal Cannulation
Tracheal Cannulation - Related Videos
02:24
Tracheal Cannulation in Rabbit Model: A Procedure to Insert Tracheal Cannula into the Rabbit Trachea
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2025
This video demonstrates the surgical creation of an opening in the rabbit trachea to insert a tracheal cannula for artificially ventilating the lungs.
04:50
Direct Tracheal Instillation of Solutes into Mouse Lung
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Cited by 54 •
2010
Intratracheal instillations deliver solutes directly into the lungs. This procedure targets the delivery of the instillate into the distal regions of the lung, and is therefore often incorporated in studies aimed at studying alveoli. We provide a detailed survival protocol for performing intratracheal instillations in mice.
02:05
Intraduodenal Cannulation in a Rat Model for Immunopathology Studies
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2025
This video demonstrates intraduodenal cannulation in a rat model. After anesthetizing the rat, the duodenum is located and a small puncture is created. A J-shaped cannula with a beveled tip is carefully inserted and secured, and the incision is closed. The cannulated rat model enables intra-intestinal infusion for immunopathology studies.
10:20
In vitro Measurements of Tracheal Constriction Using Mice
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Cited by 15 •
2012
Transgenic mice have been extremely useful in ascribing physiological function to genes. As such, research in general, and functional studies of airway, in particular, have undergone a remarkable shift toward murine models. Here we provide protocols for in vitro trachea constriction studies to evaluate smooth muscle function in murine airway.
05:18
Cannulation of the Mouse Submandibular Salivary Gland via the Wharton's Duct
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Cited by 17 •
2011
A protocol for the cannulation of the mouse submandibular salivary gland via the Wharton's duct is described. For this experiment, the trypan blue solution is used as a dyer to demonstrate how this technique effectively delivers infusions into the targeted gland, and to suggest the reliability of this new approach as a potential clinical drug/cell therapy for the regeneration of salivary glands.
FAQs
Placement within the tracheal lumen creates a stable route between the airway and the respiratory system. Maintaining patency prevents the access pathway from being lost during an experiment, while the same route can support ventilation or deliver a controlled amount of test material. This dual function is useful when airway access must remain consistent across measurements.
Controlled access matters because pulmonary experiments depend on exposing models to a defined test material. Tracheal cannulation reduces variability in how tumor cells, drugs, aerosols, or other substances reach the lungs. More consistent administration helps investigators distinguish biological differences, such as tumor development or treatment response, from differences caused by uneven delivery.
Tracheal cannulation can serve two different experimental functions: maintaining airway access for ventilation or creating a controlled route for substances delivered to the lungs. These functions are related but not interchangeable. Ventilation provides respiratory support, whereas delivery establishes exposure to a test material. Recognizing the distinction helps researchers match cannula use to the study objective.
In a cancer-research workflow, the subject is placed under anesthesia, and the cannula is guided into the tracheal lumen. Once airway patency is maintained, investigators can use the established access for controlled delivery or respiratory-sample collection. Keeping these functions within one access route supports consistent handling across studies of lung tumors, treatment response, and therapeutic safety.
The technique can support administration of tumor cells, drugs, aerosols, and other test materials to the lungs. The choice depends on the research question, such as establishing a pulmonary tumor model, evaluating a treatment, or assessing safety. Because delivery occurs through a direct, controlled airway route, investigators can apply the selected material with less dosing variability.
Respiratory samples provide an additional use beyond administering an experimental material. Cannulation can support collection from the respiratory system while the study examines pulmonary disease or treatment effects. In cancer research, combining controlled exposure with respiratory sampling can help connect the delivered intervention to observations about lung tumor development, response to therapy, or potential therapeutic safety.