Uterine Fat Isolation
Uterine Fat Isolation - Related Videos
09:25
Transplantation Into the Mouse Ovarian Fat Pad
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Cited by 7 •
2016
We describe an ovarian fad pad transplantation assay suitable for studies of normal and transformed epithelia of the female reproductive tract. The mouse fat pad allows transplantation of large tissue fragments, is easily accessible for surgery and imaging, and provides the most favorable native environment for tissues of Müllerian origin.
03:53
Ovarian Fat Pad Transplantation Assay: An In Vivo Technique to Introduce Cells into Ovarian Fat Pad in Mouse Models
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2023
In this video, we demonstrate an assay for transplantation of experimental cells and tissue fragments into the mouse ovarian fat pad. This method can be used to monitor cell regeneration and tumor transformation via non-invasive techniques.
08:02
Mouse Model of Surgically-induced Endometriosis by Auto-transplantation of Uterine Tissue
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Cited by 62 •
2012
A description of the surgical induction of endometriosis in mice and rats by auto-transplantation of uterine tissue to the arterial cascade of the intestinal mesentery.
06:30
An ex vivo Mouse Model for High-Fat Diet-Like Adipose Tissue Inflammation
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2026
Using an ex vivo explant model, small pieces of the rostral portion of visceral white adipose tissue from lean mice are cultured for 7–10 days, enabling the induction and study of adipose tissue inflammation as it occurs during high-fat diet feeding.
03:41
Harvesting Peritoneal Fat Depots: A Technique to Study Ovarian Cancer Colonization of Peritoneal Adipose Tissues in Mouse Models
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2023
In this video, we use a euthanized female mouse model bearing ovarian cancer. We then describe a protocol to identify and extract fat depots or adipose tissues associated with the five primary peritoneal organs to study metastatic cancer cell colonization.
FAQs
Mechanical mincing breaks the tissue into smaller pieces, increasing access to cells embedded within the adipose matrix. Enzymatic digestion then helps release cellular components for downstream separation. Using both steps supports recovery of distinct populations rather than analyzing the tissue as an undifferentiated mass, which is important when studying how individual cellular compartments participate in tumor–adipose interactions.
Mature adipocytes and the stromal vascular fraction represent different cellular sources of biological activity. The stromal vascular fraction contains immune, endothelial, and precursor cells, while mature adipocytes provide a separate population for analysis. Examining these fractions independently can help associate inflammatory signals, adipokines, or metabolic changes with particular cellular components of the uterine fat environment.
Adipokines and inflammatory signals can help explain communication between uterine-associated adipose tissue and nearby cancer cells. Their analysis may reveal how the local environment influences cancer cell growth or invasion. Studying these signals alongside altered metabolism provides a broader view of tumor–adipose interactions than examining cancer cells without their surrounding tissue context.
Uterine fat may affect the cancer microenvironment through coordinated cellular and molecular processes involving adipocytes, immune cells, endothelial cells, and precursor cells. These components can be examined for relationships with cancer cell growth, invasion, inflammatory activity, and metabolism. This perspective helps connect tissue composition with the behavior of tumors in their local reproductive-tissue setting.
A typical workflow begins with careful dissection of adipose tissue associated with the uterus, followed by mechanical mincing and enzymatic digestion. The resulting material is processed by filtration or centrifugation to recover mature adipocytes and the stromal vascular fraction. These separated materials can then support molecular profiling, culture experiments, or further cancer-focused analyses.
Isolated material can provide cellular and tissue-associated inputs for molecular profiling, culture experiments, and evaluation of potential therapeutic targets. Researchers can examine adipocytes or stromal vascular cells independently, or investigate their relationship to cancer cells. These applications help assess signaling, inflammatory activity, and metabolism within tumor–adipose interactions relevant to cancer biology.
Separating uterine-associated adipose tissue into cellular fractions gives researchers more specific material than an untreated tissue sample. The approach allows mature adipocytes and stromal vascular cells to be considered as distinct contributors to the cancer microenvironment. This can clarify which cellular populations may be associated with altered signaling, metabolism, cancer cell growth, or invasion.