Electropherogram
Electropherogram - Related Videos
18:17
Obtaining High Quality RNA from Single Cell Populations in Human Postmortem Brain Tissue
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Cited by 39 •
2009
We describe a process using laser-capture microdissection to isolate and extract RNA from a homogeneous cell population, pyramidal neurons, in layer III of the superior temporal gyrus in postmortem human brains. We subsequently linearly amplify (T7-based) mRNA, and hybridize the sample to the Affymetrix human X3P microarray.
08:50
Capillary Electrophoresis (CE)
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2023
Source: Laboratory of Dr. B. Jill Venton - University of Virginia Capillary electrophoresis (CE) is a separation technique that separates molecules in an electric field according to size and charge. CE is performed in a small glass tube called a capillary that is filled with an electrolyte solution. Analytes are separated due to differences in electrophoretic mobility, which varies with charge, solvent viscosity, and size. Traditional electrophoresis in gels is limited in the amount of voltage...
03:01
Detection of Fish Pathogens Using Variable Number Tandem Repeat (VNTR) Analysis
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2025
Source: Gulla, S., et al. Multi-locus Variable-number Tandem-repeat Analysis of the Fish-pathogenic Bacterium Yersinia ruckeri by Multiplex PCR and Capillary Electrophoresis. J. Vis. Exp. (2019).This video demonstrates the procedure for separating fluorescently labeled variable number tandem repeat (VNTR) amplicons, derived from a fish-pathogenic bacterium, using capillary electrophoresis to generate size-specific electropherogram profiles for bacterial identification.
04:21
Microfluidic Capillary Electrophoresis for Fragment Sizing of PCR Products: A Microchip-Based Electrophoretic Separation Technique to Separate DNA Fragments Based on Size
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2025
This video describes the microfluidic capillary electrophoresis technique to separate PCR amplicons based on their size. This technique helps in fragment sizing using fluorescent dyes and standard molecular size markers.
03:29
Capillary Electrophoresis: A Technique to Analyze Fluorescently Labeled Polymerase Chain Reaction Amplicons
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2025
This video describes the technique of capillary electrophoresis to analyze fluorescently labeled PCR amplicons obtained from various samples based on their size. It generates a migratory pattern that determines the fragments’ base-pair lengths with the help of the labeled fluorescent dye in each fragment.
FAQs
These factors influence how quickly each charged species moves through the separation medium. Differences in charge and size produce different migration velocities, while the medium contributes additional separation behavior. As a result, chemically distinct analytes can appear at different migration times or positions, allowing the resulting pattern to distinguish components within a mixture.
Peak position or migration time indicates when or where a separated species reaches the detector, whereas detector response reflects the signal produced by that species. Comparing these features helps researchers identify and compare analytes. The combined pattern is especially useful when a sample contains several components with different migration behaviors.
The separation medium is not merely a support for the sample; its properties affect analyte movement through the applied electric field. Because those properties can change migration velocities, they influence the spacing and positions of peaks. Interpreting an electropherogram therefore requires relating observed separation patterns to the medium used for the electrophoretic separation.
A sample first undergoes electrophoretic separation in a medium while an electric field drives its charged species forward. The components migrate at different velocities, creating temporal or spatial separation. A detector then records the response as species pass through the system, converting the separation into peaks that can be examined for chemical differences.
Separated peaks allow analysts to inspect whether a sample produces a simple or more complex component pattern, supporting purity assessment. Detector responses can also be used for quantitative measurements when the analytical procedure relates signal to analyte amount. Thus, the record provides both compositional information and a basis for comparing measured quantities.
In chemistry, these records support qualitative analysis, purity assessment, and quantitative measurements of separated species. They are also relevant to capillary electrophoresis and biomolecular analysis, where mixtures may contain multiple charged molecules. Examining peak positions and detector responses helps researchers evaluate the separation and interpret the chemical composition of complex samples.