Biology
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Obtaining High Quality RNA from Single Cell Populations in Human Postmortem Brain Tissue
Chapters
Summary August 6th, 2009
We describe a process using laser-capture microdissection to isolate and extract RNA from a homogeneous cell population, pyramidal neurons, in layer III of the superior temporal gyrus in postmortem human brains. We subsequently linearly amplify (T7-based) mRNA, and hybridize the sample to the Affymetrix human X3P microarray.
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