Structural Imaging
Structural Imaging - Related Videos
11:19
Imaging Subcellular Structures in the Living Zebrafish Embryo
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Cited by 8 •
2016
Imaging the dynamic behavior of organelles and other subcellular structures in vivo can shed light on their function in physiological and disease conditions. Here, we present methods for genetically tagging two organelles, centrosomes and mitochondria, and imaging their dynamics in living zebrafish embryos using wide-field and confocal microscopy.
08:00
Imaging and 3D Reconstruction of Cerebrovascular Structures in Embryonic Zebrafish
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Cited by 1 •
2014
Imaging of cerebrovascular development in larval zebrafish is described. Techniques to facilitate 3D imaging and modify cerebrovascular development using chemical treatments are also provided.
05:45
Uncovering Hidden Dynamics of Natural Photonic Structures Using Holographic Imaging
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Cited by 6 •
2022
The paper is primarily focused on the combined power of optical (linear and nonlinear) and holographic methods used to reveal phenomena at the nanoscale. The results obtained from the biophotonic and oscillatory chemical reactions' studies are given as representative examples, highlighting holography's ability to reveal dynamics at a nanoscale.
17:06
Co-analysis of Brain Structure and Function using fMRI and Diffusion-weighted Imaging
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Cited by 17 •
2012
We describe a novel approach for simultaneous analysis of brain function and structure using magnetic resonance imaging (MRI). We assess brain structure with high-resolution diffusion-weighted imaging and white-matter fiber tractography. Unlike standard structural MRI, these techniques allow us to directly relate anatomical connectivity to functional properties of brain networks.
03:26
Analyzing Amyloid Structures in a Tissue Section Using Fluorescence Lifetime Imaging Microscopy
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2025
Source: Nyström, S., et al., Imaging Amyloid Tissues Stained with Luminescent Conjugated Oligothiophenes by Hyperspectral Confocal Microscopy and Fluorescence Lifetime Imaging. J. Vis. Exp. (2017).This video demonstrates analyzing amyloid structures with fluorescence lifetime imaging microscopy or FLIM, using hFTAA dye to reveal compact cores as blue/green and unstable peripheries as red/yellow in color-coded images, reflecting amyloid organization.
FAQs
Contrast arises from how a sample interacts with the probing energy or particles used in the experiment. X-rays, electrons, and radiofrequency fields produce signals that vary with the sample’s spatial organization. Differences in those signals allow researchers to distinguish structural features and convert otherwise invisible arrangements into measurable images or models.
The detected response from a sample is processed to reveal spatial relationships among its components. Depending on the experiment, the result may be an image or a three-dimensional model that represents atoms, molecules, materials, or larger chemical structures. This reconstruction makes structural organization available for comparison, identification, and interpretation.
The probing method determines which sample interactions generate measurable contrast, so different approaches can emphasize different structural scales or chemical settings. X-ray, electron, and radiofrequency-based methods are therefore not interchangeable. Researchers select among them according to whether they need information about molecular arrangements, crystal structures, materials, surfaces, or interfaces.
Spatial arrangement connects a chemical structure with the way it behaves and functions. Structural imaging can reveal organization that helps explain reactivity, identify molecules, and relate material architecture to controlled properties. In chemistry, this connection is valuable because composition alone may not show how arrangement influences catalysts, medicines, or engineered materials.
A study begins by choosing an experimental method and probe suited to the chemical structure or material being examined. The sample is then measured through its interaction with the probe, and the resulting signals are converted into an image or three-dimensional model. Researchers interpret that output to identify structures, determine organization, or characterize a material.
Structural imaging is useful when a chemical investigation requires the spatial arrangement within a crystal rather than only its composition or identity. Experimental measurements can provide structural information that supports crystal-structure determination and comparison among chemical systems. The resulting arrangement helps researchers connect molecular organization with chemical behavior and evaluate how structure relates to function.
In catalyst research, structural information can help explain reactivity; in medicine-related chemistry, it supports molecular identification and structural analysis; and in materials research, it characterizes bulk structures, surfaces, and interfaces. These applications use the measured organization of chemical systems to guide optimization, understand performance, and design materials with controlled properties.