Three-way Syringe Coupler
Three-way Syringe Coupler - Related Videos
02:28
Extraction of Apoplastic Bacteria Using a Syringe-Based Pressure Method
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2025
Source: Rufián, J. S., et al, Single-Cell Analysis of the Expression of Pseudomonas syringae Genes within the Plant Tissue. J. Vis. Exp. (2022)This video demonstrates a syringe-based pressure method for extracting apoplastic bacteria, which proliferate and form microcolonies within the apoplast, the intercellular space between plant cells. The method yields debris-free bacterial samples suitable for downstream single-cell analysis.
09:58
Syringe-injectable Mesh Electronics for Stable Chronic Rodent Electrophysiology
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Cited by 31 •
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Mesh electronics probes seamlessly integrate and provide stable, long-term, single-neuron level recording within the brain. This protocol uses mesh electronics for in vivo experiments, involving the fabrication of mesh electronics, loading into needles, stereotaxic injection, input/output interfacing, recording experiments, and histology of tissue containing mesh probes.
05:49
Modeling the Effects of Hemodynamic Stress on Circulating Tumor Cells using a Syringe and Needle
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Cited by 2 •
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Here we demonstrate a method to apply fluid shear stress to cancer cells in suspension to model the effects of hemodynamic stress on circulating tumor cells.
09:08
Three-dimensional Printing of Thermoplastic Materials to Create Automated Syringe Pumps with Feedback Control for Microfluidic Applications
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Cited by 3 •
2018
Here we present a protocol to construct a pressure-controlled syringe pump to be used in microfluidic applications. This syringe pump is made from an additively manufactured body, off-the-shelf hardware, and open-source electronics. The resulting system is low-cost, straightforward to build, and delivers well-regulated fluid flow to enable rapid microfluidic research.
22:00
Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases
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Cited by 48 •
2010
Herein is described the procedure implemented in the Caffrey Membrane Structural and Functional Biology Group to set up manually crystallization trials of membrane proteins in lipidic mesophases.
FAQs
Plunger movement creates the pressure difference that drives liquid through the connected pathways. Pressing one syringe can push its contents toward a selected destination, while movement in another syringe can receive or draw liquid within the setup. Coordinating these actions lets researchers control transfers between connected syringes without treating each movement as a separate disconnected operation.
The three-port junction provides a shared connection point between the syringe bodies, allowing liquid to be directed between selected parts of the setup. This arrangement supports more than a single pairwise transfer because researchers can coordinate multiple syringes around one interface. In chemistry workflows, that organization is useful when reagents must be introduced, displaced, or combined in a controlled sequence.
Keeping the syringes connected maintains a continuous fluid-handling arrangement while transfers or exchanges occur. Researchers can manipulate the plungers through the same junction rather than repeatedly interrupting the setup to change connections. This can simplify small-scale operations and support more consistent coordination during sequential reagent addition, liquid exchange, or mixing within a chemistry procedure.
A general workflow is to connect the syringe bodies through the three-port junction, place the liquids in the intended syringes, and use plunger movement to drive transfer through selected pathways. The operator coordinates which syringe supplies liquid and which receives it, adjusting the sequence to the task. This arrangement keeps small-volume handling organized during the procedure.
For sequential addition, different reagents can be arranged in connected syringes and introduced through coordinated plunger movements. The junction allows the operator to move selected liquids into the working pathway in an intended order without repeatedly disconnecting syringes. This is relevant to small-scale chemical synthesis, where controlled reagent delivery can help organize the handling sequence.
The connected syringes can support liquid exchange by moving material from one syringe toward another through the shared junction. Coordinated transfers can also combine liquids within the setup, providing a small-scale mixing approach. These capabilities make the connector relevant to sample preparation and other benchtop chemistry procedures that require controlled movement or combination of liquids.