3d Tissue Culture
3d Tissue Culture - Related Videos
11:12
Tissue Engineering of a Human 3D in vitro Tumor Test System
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Cited by 34 •
2013
Methods to create human 3D tumor tissues as test systems are described. These technologies are based on a decellularized Biological Vascularized Scaffold (BioVaSc), primary human cells and a tumor cell line, which can be cultured under static as well as under dynamic conditions in a flow bioreactor.
09:35
Histotypic Tissue Culture
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2023
Although two-dimensional tissue culture has been common for some time, cells behave more realistically in a three-dimensional culture, and more closely mimics native tissue. This video introduces histotypic tissue culture, where the growth and propagation of one cell line is done in an engineered three-dimensional matrix to reach high cell density. Here, we show the harvesting of cells from donor tissue, followed by cell culture on an engineered construct.
05:47
Generation of Human 3D Lung Tissue Cultures (3D-LTCs) for Disease Modeling
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Cited by 46 •
2019
Here, we present a protocol for the preparation of agarose-filled human precision-cut lung slices from resected patient tissue that are suitable for generating 3D lung tissue cultures to model human lung diseases in biological and biomedical studies.
09:50
Impact Assessment of Repeated Exposure of Organotypic 3D Bronchial and Nasal Tissue Culture Models to Whole Cigarette Smoke
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Cited by 41 •
2015
The aim of this protocol is to expose human organotypic 3D bronchial and nasal tissue models to mainstream cigarette smoke (CS) at the air-liquid interface. The impact of CS on the tissues is then investigated using a cytochrome P450 activity assay, a cilia beating measurement, and a systems biology approach.
04:41
Magnetically Bioprinted 3D Spheroid Co-cultures: A Method for Co-culturing Cancerous Cells and Fibroblasts
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2023
This video explains the 3D magnetic bioprinting of co-cultured pancreatic cancer cells and fibroblasts. Pancreatic cancer cells and activated pancreatic fibroblast cells are co-cultured and magnetized using a biocompatible nanoparticle assembly, to generate spheroids, under the influence of magnetic forces.
FAQs
Spatial organization allows cells to retain local relationships with neighboring cells and surrounding matrix, while chemical gradients create differences across the developing tissue. Together, these features influence signaling, tissue organization, and differentiation in ways that depend on position. This makes the system useful for examining how local conditions contribute to morphogenesis and other developmental outcomes.
Supportive matrices and scaffolds provide the physical context in which cells interact with one another and with their surroundings. Those interactions can guide organization, signaling, and differentiation rather than merely serving as structural support. Selecting an appropriate three-dimensional context therefore matters when the experiment aims to connect cellular behavior with tissue architecture or developmental change.
Compared with conventional two-dimensional cultures, 3D tissue culture preserves spatial relationships and local chemical gradients more effectively. Cells therefore encounter a context that more closely reflects tissue architecture, which can alter signaling, organization, and differentiation. This added spatial information helps researchers interpret developmental processes and may provide a more physiologically relevant basis for disease modeling and drug testing.
Cell-cell interactions transmit information between neighboring cells, while cell-matrix interactions connect those signals to the surrounding scaffold or matrix. Their combined effects help determine how cells organize and differentiate within the culture. Examining both interaction types is important in developmental studies because tissue-level patterns cannot be explained solely by isolated cellular responses.
A basic setup begins by either embedding cells within a supportive matrix or scaffold or aggregating them so they can organize in three dimensions. The selected arrangement should preserve relevant cell-cell and cell-matrix interactions. Researchers can then examine resulting tissue organization, signaling, and differentiation in relation to the experimental developmental question.
3D tissue culture can reveal changes in tissue organization, signaling, and differentiation, allowing investigators to relate cellular mechanisms to larger developmental outcomes. In developmental biology, the approach supports studies of tissue formation, morphogenesis, and organ development. Its value lies in connecting molecular or cellular behavior with the spatial patterns that emerge as tissues develop.
The same spatial and interaction features that support developmental studies also make these cultures useful beyond normal tissue formation. Researchers can apply them to model disease-related tissue behavior, evaluate drugs in a more physiologically relevant platform, and investigate regenerative research questions. These applications extend the method from describing development to testing how tissues respond in structured environments.