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Generation of Human 3D Lung Tissue Cultures (3D-LTCs) for Disease Modeling
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JoVE Journal Medicine
Generation of Human 3D Lung Tissue Cultures (3D-LTCs) for Disease Modeling

Generation of Human 3D Lung Tissue Cultures (3D-LTCs) for Disease Modeling

DOI:
10.3791/58437-v

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05:47 min

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February 12, 2019

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, , , , ,
1Comprehensive Pneumology Center,Ludwig-Maximilians-Universität and Helmholtz Zentrum Munich, 2German Center of Lung Research (DZL), 3Translational Lung Research and CPC-M bioArchive, Helmholtz Zentrum München,Comprehensive Pneumology Center Munich DZL/CPC-M, 4Department of Experimental Medical Science, Lung Bioengineering and Regeneration,Lund University, 5Wallenberg Center for Molecular Medicine,Lund University, 6Stem Cell Centre,Lund University, 7Asklepios Fachkliniken Munich-Gauting, 8Department of Medicine, Division of Pulmonary Sciences and Critical Care Medicine,University of Colorado

Chapters

  • 00:04Title
  • 00:49Lung Tissue Inflation
  • 02:33Precision-cut Lung Slicing (PCLS) and PCLS Punch Generation
  • 04:02Results: Representative Experimental 48 h Human 3D-lung Tissue Culture Readouts
  • 04:55Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a protocol for the preparation of agarose-filled human precision-cut lung slices from resected patient tissue that are suitable for generating 3D lung tissue cultures to model human lung diseases in biological and biomedical studies.

Tags

3D-LTCsDisease ModelingEx VivoAgaroseBronchusCanulaPrecision Cut Lung SlicingVibratome

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