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Impact Assessment of Repeated Exposure of Organotypic 3D Bronchial and Nasal Tissue Culture Models to Whole Cigarette Smoke
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Impact Assessment of Repeated Exposure of Organotypic 3D Bronchial and Nasal Tissue Culture Models to Whole Cigarette Smoke

Impact Assessment of Repeated Exposure of Organotypic 3D Bronchial and Nasal Tissue Culture Models to Whole Cigarette Smoke

DOI:
10.3791/52325-v

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09:50 min

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February 12, 2015

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, , , , , , , , , , , , ,
1Biological Systems Research, Philip Morris International R&D,Philip Morris Products S.A.

Chapters

  • 00:05Title
  • 01:28Culture of Organotypic Bronchial Tissue
  • 02:04In vitro Cigarette Smoke (CS) Exposure
  • 03:22Measurement of Ciliary Beating & Transepithelial Electrical Resistance (TEER)
  • 04:20Cytochrome P450 (CYP) 1A1/1B1 Activity
  • 05:02RNA Extraction
  • 06:35Microarray Workflow & Washing and Staining
  • 08:07Results: Applicability of Human Organotypic Tissue Models
  • 09:03Conclusion

Summary

Automatic Translation

Automatic Translation

The aim of this protocol is to expose human organotypic 3D bronchial and nasal tissue models to mainstream cigarette smoke (CS) at the air-liquid interface. The impact of CS on the tissues is then investigated using a cytochrome P450 activity assay, a cilia beating measurement, and a systems biology approach.

Tags

Cigarette Smoke ExposureImpact AssessmentCiliary Beating FrequencyCytochrome P450 ActivityGene ExpressionSystems BiologyXenobiotic Metabolism

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