Autologous Mitochondrial Transplantation
Autologous Mitochondrial Transplantation - Related Videos
08:58
Matrix-assisted Autologous Chondrocyte Transplantation for Remodeling and Repair of Chondral Defects in a Rabbit Model
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Cited by 5 •
2013
An experimental technique for the treatment of chondral defects in the rabbit's knee joint is described. The implantation of autologous chondrocytes seeded on a matrix is a well-accepted method for the remodeling and repair of articular cartilage lesions providing satisfying long-term results. Matrix-assisted autologous chondrocyte transplantation (MACT) offers a standardized and clinically established implantation method.
10:44
Modeling Intracerebral Hemorrhage in Mice: Injection of Autologous Blood or Bacterial Collagenase
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Cited by 60 •
2012
Clinically relevant animal models of intracerebral hemorrhage (ICH) are needed to extend our knowledge of hemorrhagic stroke and to examine novel therapeutic strategies. In this study, we describe and evaluate two ICH models that implement unilateral injections of either autologous whole blood or bacterial collagenase into the basal ganglia (corpus striatum) of mice.
08:15
Cervical Heterotopic Mouse Heart Transplantation using End-to-side Anastomosis Technique to Study Transplant Immunology
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2026
This protocol presents a simplified, end-to-side suture anastomosis technique for murine cervical heart transplantation, providing a robust and reproducible model for studying transplant immunology.
10:15
Steps for the Autologous Ex vivo Perfused Porcine Liver-kidney Experiment
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Cited by 2 •
2013
Study of the animal organ physiology can be achieved by performing an experimental ex vivo perfusion system. The addition of a porcine kidney, as a homeostatic organ to our previously developed ex vivo liver perfusion model can be a principal step to achieve a better physiological environment.
02:59
Animal Mitochondrial Genetics
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2020
Among all the organelles in an animal cell, only mitochondria have their own independent genomes. Animal mitochondrial DNA is a double-stranded, closed-circular molecule with around 20,000 base pairs. Mitochondrial DNA is unique in that one of its two strands, the heavy, or H, -strand is guanine rich, whereas the complementary strand is cytosine rich and called the light, or L, -strand. Compared to nuclear DNA, mitochondrial DNA has a very low percentage of non-coding regions and is marked by...
FAQs
After delivery, mitochondria can be internalized by recipient cells and contribute to oxidative phosphorylation, the process associated with cellular ATP production. This added mitochondrial function may improve the energy available for cellular activities and support recovery when recipient cells have impaired mitochondrial performance. The resulting bioenergetic change helps researchers connect organelle transfer with functional restoration.
The approach depends on obtaining mitochondria from a healthy or accessible tissue and transferring them to cells with impaired function. This makes the quality and functional state of the source tissue central to the procedure’s rationale. Comparing source and recipient conditions allows researchers to examine whether transferred organelles can support recovery in cells with reduced mitochondrial capacity.
In developmental biology, the technique can be used to examine how mitochondrial energy metabolism affects cell survival, differentiation, and early development. These processes require researchers to consider cellular function beyond mitochondrial activity alone. By linking altered bioenergetic capacity with developmental outcomes, transplantation provides a way to study how organelle function contributes to developmental cell behavior.
A typical workflow includes obtaining mitochondria from a healthy or accessible tissue, isolating and purifying the organelles, and delivering them into recipient cells with impaired mitochondrial function. Subsequent analysis can determine whether the mitochondria were internalized and whether cellular function changed. This sequence separates preparation, delivery, and outcome assessment within the experimental procedure.
Researchers can assess whether recipient cells show evidence of mitochondrial internalization, improved oxidative phosphorylation, increased ATP production, or broader cellular recovery. These outcomes connect the physical transfer of organelles with functional consequences. Together, they help determine whether transplantation merely delivers mitochondria or also improves the bioenergetic capacity of cells.
The technique informs regenerative strategies by examining whether mitochondrial transfer can contribute to tissue repair and restoration of bioenergetic capacity. In developmental research, it provides a tool for relating mitochondrial metabolism to survival, differentiation, and early development. It also supports investigation of mitochondrial dysfunction by connecting impaired organelle activity with cellular and tissue-level consequences.