Gastrulation Neurulation

Gastrulation and neurulation are successive embryonic processes that establish the body plan and nervous system during early development. During gastrulation, coordinated cell movements such as invagination, involution, and ingression reorganize the blastula into the three germ layers: ectoderm, mesoderm, and endoderm. Neurulation then converts a region of ectoderm into the neural plate, whose edges elevate, bend, and fuse to form the neural tube, the precursor to the brain and spinal cord. Together, these processes establish tissue organization, body axes, and organ-forming potential, making them central to developmental biology and to research on congenital abnormalities, stem-cell models, and regenerative medicine.

Gastrulation Neurulation - Related Videos

Education

JoVE Core - Biology

Neurulation

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2025

Neurulation is the embryological process which forms the precursors of the central nervous system and occurs after gastrulation has established the three primary cell layers of the embryo: ectoderm, mesoderm, and endoderm. In humans, the majority of this system is formed via primary neurulation, in which the central portion of the ectoderm—originally appearing as a flat sheet of cells—folds upwards and inwards, sealing off to form a hollow neural tube. As development proceeds, the anterior...

Gastrulation

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2025

Gastrulation establishes the three primary tissues of an embryo: the ectoderm, mesoderm, and endoderm. This developmental process relies on a series of intricate cellular movements, which in humans transforms a flat, “bilaminar disc” composed of two cell sheets into a three-tiered structure. In the resulting embryo, the endoderm serves as the bottom layer, and stacked directly above it is the intermediate mesoderm, and then the uppermost ectoderm. Respectively, these tissue strata will form...

Research

JoVE Journal - Developmental Biology

Single-Cell RNA Sequencing of Mutant Whole Mouse Embryos: From the Epiblast to the End of Gastrulation

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Cited by 4 •

2024

This paper establishes a pipeline for high-quality single-cell and nuclei suspensions of gastrulating mouse embryos for sequencing of single cells and nuclei.

Imaging of Cell Shape Alteration and Cell Movement in Drosophila Gastrulation Using DE-cadherin Reporter Transgenic Flies

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2016

Herein we describe a procedure to capture live images of Drosophila gastrulation. This has enabled us to better understand the apical constriction involved in early development and further analyze mechanisms governing cellular movements during tissue structure modification.

Research

JoVE Journal - Developmental Biology
Free Sample

Visualizing the Node and Notochordal Plate In Gastrulating Mouse Embryos Using Scanning Electron Microscopy and Whole Mount Immunofluorescence

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Cited by 10 •

2018

The node and notochordal plate are transient signaling organizers in developing mouse embryos that can be visualized using several techniques. Here, we describe in detail how to perform two of the techniques to study their structure and morphogenesis: 1) scanning electron microscopy (SEM); and 2) whole mount immunofluorescence (WMIF).

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