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Visualizing the Node and Notochordal Plate In Gastrulating Mouse Embryos Using Scanning Electron Microscopy and Whole Mount Immunofluorescence
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Visualizing the Node and Notochordal Plate In Gastrulating Mouse Embryos Using Scanning Electron Microscopy and Whole Mount Immunofluorescence

Visualizing the Node and Notochordal Plate In Gastrulating Mouse Embryos Using Scanning Electron Microscopy and Whole Mount Immunofluorescence

DOI:
10.3791/58321-v

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09:36 min

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November 06, 2018

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, ,
1Graduate Program in Pharmacology and Experimental Therapeutics,University of Cologne, 2Department of Dermatology and Venereology,University Hospital of Cologne, 3Cologne Cluster of Excellence in Cellular Stress Responses in Aging-Associated Diseases (CECAD),University of Cologne, 4Department of General Ecology, Institute for Zoology, Biocenter Cologne,University of Cologne

Chapters

  • 00:04Title
  • 00:59Scanning Electron Microscopy of the Mouse Embryonic Node
  • 04:17Whole Mount Immunofluorescence of the Mouse Node and Notochordal Plate
  • 08:06Results: Strip1 Mutant Embryos Display Irregular Nodes and Abnormal F-Actin Organization in the Axial Mesoderm
  • 08:46Conclusion

Summary

Automatic Translation

Automatic Translation

The node and notochordal plate are transient signaling organizers in developing mouse embryos that can be visualized using several techniques. Here, we describe in detail how to perform two of the techniques to study their structure and morphogenesis: 1) scanning electron microscopy (SEM); and 2) whole mount immunofluorescence (WMIF).

Tags

Keywords: NodeNotochordal PlateMouse EmbryoScanning Electron Microscopy (SEM)Whole Mount ImmunofluorescenceEmbryonic DevelopmentDissectionFixationDehydrationGenotypingReichert’s MembraneYolk Sac

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