Sers Substrates
Sers Substrates - Related Videos
03:33
Fabrication of polydimethylsiloxane (PDMS)-Based Flexible Surface-Enhanced Raman Scattering (SERS) Substrate for Ultrasensitive Detection
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Cited by 2 •
2023
This protocol describes a fabrication method for a flexible substrate for surface-enhanced Raman scattering. This method has been used in the successful detection of low concentrations of R6G and Thiram.
![Quantitative SERS Detection of Uric Acid via Formation of Precise Plasmonic Nanojunctions within Aggregates of Gold Nanoparticles and Cucurbit[<em>n</em>]uril](/_next/image?url=https%3A%2F%2Fcloudfront.jove.com%2FCDNSource%2Fteasers%2F61682.jpg%3Fv%3D2026-09-01%252015%253A22%253A12&w=750&q=75)
10:02
Quantitative SERS Detection of Uric Acid via Formation of Precise Plasmonic Nanojunctions within Aggregates of Gold Nanoparticles and Cucurbit[n]uril
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Cited by 4 •
2020
A host-guest complex of cucurbit[7]uril and uric acid was formed in an aqueous solution before adding a small amount into Au NP solution for quantitative surface-enhanced Raman spectroscopy (SERS) sensing using a modular spectrometer.
11:13
Identification of Kinase-substrate Pairs Using High Throughput Screening
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Cited by 1 •
2015
Protein phosphorylation is a central feature of how cells interpret and respond to information in their extracellular milieu. Here, we present a high throughput screening protocol using kinases purified from mammalian cells to rapidly identify kinases that phosphorylate a substrate(s) of interest.
04:25
Substrate Oxidation Assay in Cultured Cells: An In Vitro Assay to Quantify Substrate Oxidation in Cells by Measuring Radioactive Signals from Trapped CO2
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2025
This video demonstrates a quantification technique for CO2 released during substrate oxidation using 14C-radiolabeled substrates. The released 14CO2 is trapped in an alkaline solution and quantified by a scintillation counter. The oxidation of different substrates varies between tissues and reflects the pathophysiological condition of the tissue.
11:53
Substrate Generation for Endonucleases of CRISPR/Cas Systems
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Cited by 4 •
2012
CRISPR/Cas systems mediate adaptive immunity in Bacteria and Archaea. Many Cas proteins are proposed to act as endoribonucleases acting on crRNA precursors of varying length. Here we illustrate three different approaches to generate pre-crRNA substrates for the biochemical analysis of Cas endonuclease activity.
FAQs
Nanostructure geometry and spacing shape the local electromagnetic field produced by localized surface plasmon resonances. Engineers adjust these features to concentrate incident light near the surface, where nearby molecules generate a stronger Raman response. Design evaluation therefore considers both enhancement and spatial consistency, since a substrate that produces a high signal but varies across locations may be less useful analytically.
The electromagnetic contribution arises when incident light excites localized surface plasmon resonances in gold or silver nanostructures. A second, chemical contribution can occur through close contact between the analyte and metal surface. Distinguishing these contributions helps engineers determine whether geometry, material composition, or analyte-surface interaction requires optimization for the intended sensing application.
Surface chemistry influences which analytes remain on or near the engineered metal surface, making it central to selectivity. By designing this interface alongside the nanostructure, engineers can favor detection of particular chemical species rather than relying only on stronger optical enhancement. This approach is especially relevant when a platform must distinguish target molecules during trace analysis or sensing.
Material composition determines the plasmonic platform used for enhancement. Gold and silver are the principal nanostructured materials identified for SERS substrates, and their role is to support localized surface plasmon resonances under illumination. Comparing composition with geometry and surface chemistry allows engineers to optimize the combined optical response, molecular interaction, and selectivity of a substrate.
An engineering workflow starts by choosing the metal, nanostructure geometry, feature spacing, and surface chemistry. The analyte is then positioned on or near the engineered surface so that illumination can interact with the nanostructure and molecule. Measurements are evaluated through Raman signal intensity, reproducibility, and selectivity, linking design choices to analytical performance.
Researchers select SERS substrates when they need highly sensitive chemical information from small amounts of material. Supported uses include trace analysis, materials characterization, environmental monitoring, biomedical sensing, and compact analytical devices. The design emphasis depends on the use case: signal strength may dominate trace detection, whereas reproducibility, selectivity, or miniaturization may matter more for device development.
Within engineering, SERS substrates are treated as tunable interfaces rather than fixed sensing surfaces. Geometry, composition, spacing, and surface chemistry can be coordinated to balance stronger Raman signals, consistent measurements, and selective molecular response. This design perspective connects nanoscale optical behavior with practical platform development, including compact analytical devices and application-specific sensing.