Transgenic Flies
Transgenic Flies - Related Videos
10:29
The FlyBar: Administering Alcohol to Flies
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Cited by 15 •
2014
Drosophila has emerged as a significant model system for dissecting the cellular and molecular underpinnings of behavioral responses to alcohol. Here we present a protocol for the collection of alcohol sensitivity data in a circadian context that can be easily applied to other experiments and is well-suited for undergraduate research.
11:15
A Simple Way to Measure Ethanol Sensitivity in Flies
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Cited by 33 •
2011
A simple assay to measure the sedating effects of ethanol on Drosophila flies, based on the loss of righting reflex, is described.
11:06
Studying Aggression in Drosophila (fruit flies)
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Cited by 19 •
2007
Aggression is an innate behavior that evolved in the framework of defending or obtaining resources. This complex social behavior is influenced by ...
17:39
Microinjection of A. aegypti Embryos to Obtain Transgenic Mosquitoes
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Cited by 56 •
2007
In this video, Nijole Jasinskiene demonstrates the methodology employed to generate transgenic Aedes aegypti mosquitoes, which are vectors for dengue fever. The techniques for correctly preparing microinjection needles, dessicating embryos, and performing microinjection are demonstrated.
04:42
Imaging of Cell Shape Alteration and Cell Movement in Drosophila Gastrulation Using DE-cadherin Reporter Transgenic Flies
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2016
Herein we describe a procedure to capture live images of Drosophila gastrulation. This has enabled us to better understand the apical constriction involved in early development and further analyze mechanisms governing cellular movements during tissue structure modification.
FAQs
Regulatory elements determine when and where an introduced sequence is expressed, rather than simply whether the sequence is present. This lets researchers connect gene activity with a particular developmental stage, tissue, or observable trait. In genetics experiments, altering expression patterns can therefore help distinguish a gene’s effects from the consequences of carrying the DNA without its regulated activity.
Transmission through the germline makes the genetic change available in offspring, allowing investigators to establish inheritance across generations. Researchers can then perform controlled crosses and compare descendants that differ in the introduced sequence or its expression. This inherited design supports mutation studies and helps separate effects associated with a transgene from variation observed in an individual fly.
The introduced sequence can be used to study protein function by observing traits associated with its expression. Coupling this approach with regulatory control enables researchers to examine effects in selected biological contexts. This makes flies useful for relating molecular changes to development, behavior, or disease mechanisms, while maintaining a genetic framework for controlled comparisons.
Researchers begin by introducing a DNA sequence into the Drosophila genome, incorporating regulatory elements when controlled expression is needed. They then follow whether the sequence is expressed in the relevant pattern and track its transmission through the germline to offspring. Finally, observable traits can be compared with the genetic change to connect the manipulation with biological outcomes.
Because the flies can connect introduced genetic changes with observable traits, they support studies of development, behavior, disease mechanisms, and protein function. The same model can therefore serve both phenotype-focused work, such as examining behavior, and mechanism-focused work, such as relating a modified sequence to protein activity. Its value lies in linking genetic design to biological outcome.
Short generation times allow researchers to obtain successive generations efficiently, while the well-characterized genome supports interpretation of genetic changes. Efficient genetic tools also enable controlled crosses, mutation studies, and high-throughput screening. Together, these features make the model suitable for experiments that require systematic comparisons across many genetic conditions or observable outcomes.