Conidia Isolation
Conidia Isolation - Related Videos
09:43
Measuring Phagocytosis of Aspergillus fumigatus Conidia by Human Leukocytes using Flow Cytometry
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Cited by 2 •
2019
This protocol provides a fast and reliable method to quantitatively measure phagocytosis of Aspergillus fumigatus conidia by human primary phagocytes using flow cytometry and to discriminate phagocytosis of conidia from mere adhesion to leukocytes.
07:21
Viability Assay of Trichoderma stromaticum Conidia Inside Human Peripheral Blood Mononuclear-Derived Macrophages
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Cited by 1 •
2023
The technique involving the phagocytosis of fungal conidia by macrophages is widely used for studies evaluating the modulation of the immune responses against fungi. The purpose of this manuscript is to present a method for evaluating the phagocytosis and clearance abilities of human peripheral blood mononuclear-derived macrophages stimulated with Trichoderma stromaticum conidia.
15:01
Confocal Laser Scanning Microscopy-Based Quantitative Analysis of Aspergillus fumigatus Conidia Distribution in Whole-Mount Optically Cleared Mouse Lung
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Cited by 3 •
2021
We describe the method for quantitative analysis of the distribution of Aspergillus fumigatus conidia (3 µm in size) in the airways of mice. The method also can be used for the analysis of microparticles and nanoparticle agglomerate distribution in the airways in various pathological condition models.
12:29
Live Imaging of Antifungal Activity by Human Primary Neutrophils and Monocytes in Response to A. fumigatus
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Cited by 10 •
2017
Here, we describe a protocol to assess antifungal activity of primary human immune cells in real-time using fluorescent Aspergillus reporter conidia in conjunction with live-cell video microscopy and flow cytometry. Generated data provide insight into host cell-Aspergillus interactions such as fungicidal activity, phagocytosis, cell migration and inhibition of fungal growth.
08:09
Vasodilation of Isolated Vessels and the Isolation of the Extracellular Matrix of Tight-skin Mice
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Cited by 1 •
2017
We describe the isolation of cardiac extracellular matrix from C57Bl/6J control mice, tight-skin mice, and tight-skin mice treated with the IRF5 inhibitory peptide. We also describe the vasodilation studies on the isolated vessels from C57Bl/6J, tight-skin mice and tight-skin mice treated with the IRF5 inhibitory peptide.
FAQs
Hyphal fragments can make a spore preparation less uniform and interfere with interpreting infection experiments. Separating these structures helps ensure that the inoculum reflects conidia rather than a mixture of fungal forms. This distinction matters when researchers compare fungal viability, germination, host-cell recognition, inflammatory signaling, or disease-related outcomes across experimental conditions.
These operations support different parts of the same separation process. Controlled agitation helps release conidia from a sporulating culture into liquid, while filtration or centrifugation helps separate them from hyphal material and other debris. Selecting and controlling these steps influences the purity and consistency of the recovered preparation, which affects downstream measurements.
A standardized conidial inoculum allows researchers to compare experiments using a more consistent fungal input. Without that consistency, differences in germination, immune recognition, inflammatory signaling, or disease development may reflect variation in the starting preparation rather than the biological condition being tested. Measurable preparations therefore strengthen reproducibility and interpretation.
Preparation quality depends on the condition of the sporulating culture, the suitability of the suspension liquid, and how carefully separation steps are controlled. Inadequate recovery or incomplete removal of debris can alter the composition of the inoculum. These differences may affect apparent viability, fungal behavior, and host responses in subsequent experiments.
The workflow begins with a culture that is producing conidia. Spores are harvested and suspended in a suitable liquid, then the suspension is processed using controlled agitation, filtration, centrifugation, or a combination of these approaches to reduce hyphal fragments and debris. The resulting preparation can then be standardized and used as an experimental inoculum.
The essential components are a sporulating fungal culture, a suitable liquid for suspension, and equipment or procedures that provide controlled agitation and separation. Filtration and centrifugation are specifically used to help remove unwanted material. Maintaining control over these conditions supports recovery of a preparation that is both purified and measurable.
Researchers use isolated conidia when they need a defined fungal input for studying interactions with host defenses. The preparation can support experiments on fungal germination, host-cell recognition, inflammatory signaling, and disease development. Because the inoculum is standardized, investigators can compare fungal viability, virulence, and immune responses more reliably between conditions.
A consistent preparation supports measurements of whether conidia remain viable, how they germinate, and how host cells recognize them. It can also be used to examine inflammatory signaling and broader disease development. In comparative studies, the same preparation strategy helps relate observed differences to fungal virulence or immune defense rather than inconsistent inoculum composition.