Viability Assay of <em>Trichoderma stromaticum</em> Conidia Inside Human Peripheral Blood Mononuclear-Derived Macrophages

Uener Ribeiro dos Santos; Julyanna Oliveira de Castro; Melissa Erc&#237;lia Santos Matos; Giulia De Bonis; Jane Lima dos Santos

doi:10.3791/65231

Journal

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Immunology and Infection

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Viability Assay of Trichoderma stromaticum Conidia Inside Human Peripheral Blood Mononuclear-Derived Macrophages

Viability Assay of <em>Trichoderma stromaticum</em> Conidia Inside Human Peripheral Blood Mononuclear-Derived Macrophages

JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection

Viability Assay of Trichoderma stromaticum Conidia Inside Human Peripheral Blood Mononuclear-Derived Macrophages

Viability Assay of Trichoderma stromaticum Conidia Inside Human Peripheral Blood Mononuclear-Derived Macrophages

1,280 Views

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07:21 min

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October 20, 2023

DOI:

10.3791/65231-v

DOI:

10.3791/65231-v

•

07:21 min

October 20, 2023

•

3 Views

Uener Ribeiro dos Santos*¹, Julyanna Oliveira de Castro*¹, Melissa Ercília Santos Matos¹, Giulia De Bonis², Jane Lima dos Santos¹

¹Laboratory of Immunobiology – Department of Biological Sciences,State University of Santa Cruz – UESC, ²Università Degli Studi di Genova

Transcript

Our research is focused on understand the interaction between the Trichoderma fungi and the mammalian immune system. How do some Trichoderma species cause disease, how can they modulate the immune system, and how can this modulation impact the development of the disease? To answer this question, we use in vitro randomized models.

We show that Trichoderma species modulates macrophages and neutrophils by targeting receptors involved in the phagocytosis process. These receptors include toll-like receptors and lectin receptors. When there is a decrease in phagocyte capacity of immune cells, susceptibility to pathogens, especially intracellular pathogens does increase.

Ongoing, have many invasion mechanism to survive in the host. This include avoiding phagocytosis and survive inside macrophage. Pathogenic species use macrophage as Trojan horses to survive in disseminating the host.

Here, we propose that to know if Trichoderma can survive inside macrophage, and for how long they survive.

Summary

The technique involving the phagocytosis of fungal conidia by macrophages is widely used for studies evaluating the modulation of the immune responses against fungi. The purpose of this manuscript is to present a method for evaluating the phagocytosis and clearance abilities of human peripheral blood mononuclear-derived macrophages stimulated with Trichoderma stromaticum conidia.