In Vivo Treg Induction
In Vivo Treg Induction - Related Videos
15:33
Adenoviral Transduction of Naive CD4 T Cells to Study Treg Differentiation
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Cited by 7 •
2013
Adenoviral gene transfer into naive CD4 T cells with transgenic expression of the Coxsackie adenovirus receptor enables the molecular analysis of regulatory T cell differentiation in vitro.
11:33
Inductance
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2023
Source: Yong P. Chen, PhD, Department of Physics & Astronomy, College of Science, Purdue University, West Lafayette, IN This experiment will use inductive coils to demonstrate the concept of inductor and inductance. Magnetic induction will be demonstrated using a rod magnet inserted into or extracted away from the core of a coil to induce a transient electromotive force (emf) voltage in the coil, measured by a voltmeter. This experiment will also demonstrate the mutual inductance between...
10:19
Induction of an Isoelectric Brain State to Investigate the Impact of Endogenous Synaptic Activity on Neuronal Excitability In Vivo
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Cited by 3 •
2016
This procedure performs long-lasting in vivo intracellular recordings from single neurons during physiologically relevant cerebral states and after complete abolition of ongoing electrical activities, resulting in an isoelectric brain state. The physiological constants of the animal are carefully monitored during the transition to the artificial comatose condition.
03:21
Acinar-to-Ductal Metaplasia Induction: A Method to Study Acinar Cell to Ductal Cell Differentiation in 3D Ex Vivo Culture
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2023
This video describes the technique of differentiating pancreatic acinar cells to ductal cells in ex vivo 3D culture. This helps to understand the mechanisms regulating the acinar-to-ductal metaplasia (ADM), a key process in the early development of pancreatic cancer.
08:02
In Vivo Augmentation of Gut-Homing Regulatory T Cell Induction
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Cited by 1 •
2020
Here we present a protocol for in vivo augmentation of gut-homing regulatory T cell induction. In this protocol, dendritic cells are engineered to locally produce high concentrations of the active vitamin D (1,25-dihydroxyvitamin D or 1,25[OH]2D) and the active vitamin A (retinoic acid or RA) de novo.
FAQs
In vivo Treg induction depends on coordinated cues rather than antigen recognition alone. When naïve CD4+ T cells encounter antigen alongside transforming growth factor-β and interleukin-2, these signals support differentiation toward FoxP3-expressing cells. Tissue-derived factors further shape that outcome, helping explain why regulatory responses can vary across anatomical or inflammatory settings.
Tissue-derived factors help determine whether antigen recognition and cytokine signals promote differentiation into regulatory cells. Their influence adds local context to the effects of transforming growth factor-β and interleukin-2, so the same general induction mechanism may produce different regulatory outcomes during infection, vaccination, or inflammation. This tissue sensitivity is important when interpreting immune tolerance.
FoxP3 expression identifies the differentiated cell state associated with suppressive regulatory activity in this context. Monitoring FoxP3-expressing cells therefore links the initiating signals to the expected functional outcome: reduced excessive immune activation and stronger self-tolerance. This relationship helps immunologists assess whether an immune response has acquired a regulatory character rather than remaining broadly activated.
Researchers study the process in these settings to determine how tolerance develops while the immune system responds to changing challenges. The analysis focuses on whether conditions associated with antigen recognition, transforming growth factor-β, interleukin-2, and tissue-derived factors support FoxP3-expressing suppressive cells. Findings can clarify how regulatory responses accompany distinct immune states.
This research can examine how immune tolerance develops and can assess strategies intended to reduce autoimmune damage or limit harmful immune responses. It is especially relevant when inflammation becomes excessive, because regulatory cells may provide a way to understand how immune activation is constrained. The same studies also reveal potential tradeoffs between suppression and protective immunity.
Regulatory activity can shape infection outcomes because limiting immune activation may alter the balance between controlling inflammation and maintaining responses against pathogens. The overview identifies effects on pathogen persistence and disease outcomes, making this process relevant to infection research beyond autoimmunity. Studying that connection helps explain why tolerance-related mechanisms may influence the course of disease.