Mesenteric Lymph Duct Cannulation
Mesenteric Lymph Duct Cannulation - Related Videos
04:55
Mesenteric Lymph Duct Cannulation in a Rat Model to Harvest Lymph Fluid
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2025
This video demonstrates a technique to cannulate the mesenteric lymph duct in rats. This method enables direct access to the entire volume of components present in the lymph fluid.
13:17
The Mesenteric Lymph Duct Cannulated Rat Model: Application to the Assessment of Intestinal Lymphatic Drug Transport
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Cited by 48 •
2015
Here we describe a technique to cannulate the mesenteric lymph duct in rats which enables quantification of lipid and drug transport via the lymphatic system following intestinal delivery. The technique can be adapted to assess mesenteric lymph concentrations and/or transport of fluid, immune cells, peptides, proteins and lipophilic molecules.
05:18
Cannulation of the Mouse Submandibular Salivary Gland via the Wharton's Duct
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Cited by 17 •
2011
A protocol for the cannulation of the mouse submandibular salivary gland via the Wharton's duct is described. For this experiment, the trypan blue solution is used as a dyer to demonstrate how this technique effectively delivers infusions into the targeted gland, and to suggest the reliability of this new approach as a potential clinical drug/cell therapy for the regeneration of salivary glands.
11:06
Laparoscopic Technique for Serial Collection of Liver and Mesenteric Lymph Nodes in Macaques
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Cited by 16 •
2017
Here, we describe a minimally invasive laparoscopic technique for serial sampling of liver and mesenteric lymph nodes (MLN) in macaques that allows for increased sampling frequency, and reduces the potential for surgical complications when compared to performing a laparotomy.
10:48
Assessing Myogenic Response and Vasoactivity In Resistance Mesenteric Arteries Using Pressure Myography
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Cited by 24 •
2015
Pressure myography is used to assess vasoactivity of small arteries that develop sustained constriction when pressurized. This manuscript provides a detailed protocol to assess in isolated segments of small mesenteric arteries from rats, vasoactivity and the effect of intraluminal pressure on vascular diameter.
FAQs
The collected lymph can be evaluated for immune cells, antigens, lipids, microbial products, and soluble factors transported from the intestine. Examining these components together helps researchers connect intestinal conditions with the material entering lymphatic circulation, supporting analysis of antigen transport, inflammatory responses, and interactions between the gut and the immune system.
Mesenteric lymph offers a more direct view of material leaving the intestine before its effects are assessed only through circulating blood. This distinction can reveal gut-derived immune cells, antigens, lipids, and microbial products associated with local intestinal events, helping investigators identify trafficking or transport patterns that may be less apparent in blood samples.
By monitoring material transported from the gut through the mesenteric lymphatic pathway, researchers can relate intestinal exposure or inflammation to downstream dissemination. The approach is especially useful for examining how antigens, immune cells, and microbial products move away from the intestine and contribute to broader host responses during mucosal immune or infection studies.
The core workflow consists of surgically accessing a mesenteric lymphatic vessel, placing a catheter within that vessel, and collecting the lymph over time. This arrangement creates sustained access to gut-draining lymph for subsequent cellular, molecular, and soluble-component analyses, rather than limiting investigators to a single indirect measurement from another biological compartment.
Collected lymph can support cellular analysis of immune trafficking, molecular examination of transported antigens or microbial products, and assessment of soluble components associated with inflammation. Together, these measurements can characterize what leaves the intestine, how gut-derived material is distributed through lymph, and which immune processes accompany intestinal challenge or exposure.
The technique is valuable when a study needs to track intestinal immune trafficking, antigen transport, inflammatory responses, or host interactions with enteric pathogens. It also supports investigations of mucosal immunity and vaccine responses by connecting events in the gut with lymphatic dissemination, including disease mechanisms that blood sampling alone may not adequately reveal.