Perianal Swabbing

Perianal swabbing is a specimen-collection method that samples microorganisms, genetic material, or parasitic eggs from the skin surrounding the anus for laboratory analysis. A sterile swab, sometimes moistened with transport medium, is rotated across the perianal folds and then placed in a suitable container; culture, microscopy, or molecular testing can identify the target. In immunology and infection research, this approach supports detection of intestinal colonization, surveillance for antimicrobial-resistant organisms, investigation of enteric infections, and evaluation of host–microbe interactions. Because sampling is minimally invasive, repeated perianal swabs can help monitor microbial carriage and changes during infection or treatment.

Perianal Swabbing - Related Videos

Research

JoVE Journal - Immunology and Infection

Swab Sampling Method for the Detection of Human Norovirus on Surfaces

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Cited by 40 •

2017

A macrofoam based sampling methodology was developed and evaluated for the detection and quantification of norovirus on environmental hard surfaces.

Quantification of Group B Streptococcus Load in the Mouse Vaginal Lumen via Swabbing and Chromogenic Culture

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2026

Source: Patras, K. A. & Doran, K. S. A Murine Model of Group B Streptococcus Vaginal Colonization. J. Vis. Exp. (2016)This video demonstrates the quantification of vaginal Group B Streptococcus (GBS) load in a mouse model using swabbing and chromogenic culture. It outlines the steps involved in sample collection, plating, and colony counting to quantify GBS load in the mouse vaginal lumen.

Swab-based Conjunctival Commensal Bacteria Isolation: A Technique to Isolate Commensal Bacteria from Conjunctiva of Murine Model

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2025

This video describes a swab-based technique to isolate viable commensal bacteria from the ocular conjunctiva. The technique can be used to profile the commensal bacterial microbiome of the conjunctiva, which plays a defensive role against pathogens.

Research

JoVE Journal - Biology
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Swabbing the Urban Environment - A Pipeline for Sampling and Detection of SARS-CoV-2 From Environmental Reservoirs

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Cited by 5 •

2021

A citizen science project was designed to recruit San Diego residents to collect environmental samples for SARS-CoV-2. A multilingual web-based platform was created for data submission using a user-friendly mobile device interface. A laboratory information management system facilitated the collection of thousands of geographically diverse samples with real-time outcome tracking.

Education

JoVE Science Education - Clinical Skills

COVID-19 / Coronavirus Outbreak: Performing a Nasal Swab Test on Patients inside a Rapidly Deployable Facility Optimized for Epidemics

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2020

While different types of diagnostic tests may be used to detect coronavirus disease, a nasopharyngeal swab test is the most common. The test must be performed by a trained healthcare provider. If cell culture is collected in a wrong manner, the diagnosis obtained may be incorrect. The World Health Organization (WHO) recommends that providers use a sterile dacron or rayon swab with a flexible, plastic shaft for deep nasal sampling. Cotton, calcium alginate swabs, or swabs with wooden sticks may...

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