Microvascular Imaging
Microvascular Imaging - Related Videos
06:54
An Ex Vivo Method for Time-Lapse Imaging of Cultured Rat Mesenteric Microvascular Networks
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Cited by 18 •
2017
Angiogenesis involves multi-cell, multi-system interactions that need to be investigated in a physiologically relevant environment. The objective of this study is to demonstrate the ability of the rat mesentery culture model to make time-lapse comparisons of intact microvascular networks during angiogenesis.
09:04
In Vivo Tracking of Edema Development and Microvascular Pathology in a Model of Experimental Cerebral Malaria Using Magnetic Resonance Imaging
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Cited by 7 •
2017
We describe a mouse model of experimental cerebral malaria and show how inflammatory and microvascular pathology can be tracked in vivo using magnetic resonance imaging.
09:23
Isolation of Microvascular Endothelial Tubes from Mouse Resistance Arteries
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Cited by 20 •
2013
We present a preparation for visualizing and manipulating calcium signaling in native, intact microvascular endothelium. Endothelial tubes freshly isolated from mouse resistance arteries supplying skeletal muscle retain in vivo morphology and dynamic signaling within and between neighboring cells. Endothelial tubes can be prepared from microvessels of other tissues and organs.
10:35
Microvascular Decompression: Salient Surgical Principles and Technical Nuances
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Cited by 13 •
2011
There are many available options for management of the patient with trigeminal neuralgia. Microvascular decompression, while the most invasive of all options, is also the most effective at achieving long term remission of symptoms. Video instruction on how to maximize efficacy and minimize complications with this procedure is described.
11:08
Endothelialized Microfluidics for Studying Microvascular Interactions in Hematologic Diseases
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Cited by 52 •
2012
A method to culture an endothelial cell monolayer throughout the entire inner 3D surface of a microfluidic device with microvascular-sized channels (<30 μm) is described. This in vitro microvasculature model enables the study of biophysical interactions between blood cells, endothelial cells, and soluble factors in hematologic diseases.
FAQs
The approach separates relatively stable features, such as vessel arrangement and capillary-network organization, from dynamic measurements of local blood flow and perfusion. This distinction helps determine whether inflammation or infection primarily changes the physical vascular pattern, the efficiency of blood delivery, or both. Linking structural and functional findings provides a more complete assessment of tissue-level vascular responses.
Changes in permeability indicate that the vessel wall is behaving differently, while leukocyte movement shows how immune cells interact with the microcirculation. Examining these processes together connects vascular alterations with immune-cell recruitment. In infection and inflammation studies, that relationship can clarify how local immune responses contribute to tissue injury or changing tissue perfusion.
Optical and contrast-based approaches can capture complementary aspects of microvascular behavior in living tissues. Measurements may show vessel structure, capillary-network organization, local flow, altered permeability, or leukocyte movement. Using these readouts allows investigators to examine both anatomical changes and active vascular processes rather than relying only on a single indicator of tissue perfusion.
Perfusion measurements describe how blood reaches local tissue, whereas vascular function encompasses additional changes in flow behavior, permeability, and interactions with circulating immune cells. Considering these readouts together helps distinguish reduced or redistributed blood delivery from wider microcirculatory dysfunction. That distinction is useful when interpreting vascular injury caused by inflammation, infection, or therapeutic intervention.
A study can acquire images of the relevant microvascular region, measure vessel structure and local flow, and then evaluate dynamic features such as permeability or leukocyte movement. Investigators can compare these measurements across inflammatory, infectious, or treatment-related conditions. The resulting comparison connects cellular activity with changes in tissue perfusion and vascular behavior.
It is useful when researchers need to connect immune-cell responses with vascular and tissue consequences. Applications described for this context include examining inflammation, pathogen invasion, immune-cell recruitment, vascular injury, infectious-disease progression, and treatment effects. Microvascular measurements can show how these processes reshape local circulation and help relate microscopic events to tissue-level outcomes.
Measurements can reveal whether infection or an intervention is associated with changes in vessel structure, local blood flow, permeability, or leukocyte movement. These outcomes provide evidence about vascular injury, progression of infectious disease, and the effects of treatment. Interpreting several measurements together can indicate whether tissue changes reflect altered perfusion, immune recruitment, or both.