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JoVE Journal
Biology

This content is Open Access.

Isolation of Microvascular Endothelial Tubes from Mouse Resistance Arteries
 

Isolation of Microvascular Endothelial Tubes from Mouse Resistance Arteries

Article doi: 10.3791/50759
November 25th, 2013

Summary November 25th, 2013

We present a preparation for visualizing and manipulating calcium signaling in native, intact microvascular endothelium. Endothelial tubes freshly isolated from mouse resistance arteries supplying skeletal muscle retain in vivo morphology and dynamic signaling within and between neighboring cells. Endothelial tubes can be prepared from microvessels of other tissues and organs.

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