Organelle Remodeling
Organelle Remodeling - Related Videos
02:54
Nucleosome Remodeling
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2020
Nucleosomes are the basic units of chromatin compaction. Each nucleosome consists of the DNA bound tightly around a histone core, which makes the DNA inaccessible to DNA binding proteins such as DNA polymerase and RNA polymerase. Hence, the fundamental problem is to ensure access to DNA when appropriate, despite the compact and protective chromatin structure. Nucleosome remodeling complex Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...
15:39
Studying Organelle Dynamics in B Cells During Immune Synapse Formation
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Cited by 8 •
2019
Herein we describe two approaches to characterize cell polarization events in B lymphocytes during the formation of an IS. The first, involves quantification of organelle recruitment and cytoskeleton rearrangements at the synaptic membrane. The second is a biochemical approach, to characterize changes in composition of the centrosome, which undergoes polarization to the immune synapse.
03:24
Organelle Dynamics in B Lymphocytes following Activation with Antigen-Coated Beads
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2025
In this video, we describe a method to study the organelle dynamics in B lymphocytes following their activation with antigen-coated microbeads.
02:58
Nanoparticle-Mediated Visualization of Endo-Lysosomal Remodeling in Bacterially-Infected Cells
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2025
Source: Zhang, Y., et al. Application of Fluorescent Nanoparticles to Study Remodeling of the Endo-lysosomal System by Intracellular Bacteria. J. Vis. Exp. (2015)This video demonstrates the use of fluorescent nanoparticles to visualize endo-lysosomal remodeling in human epithelial cells infected by intracellular bacteria. Following bacterial invasion, the pathogens manipulate the host cytoskeleton and endo-lysosomal system, forming vacuoles with tubular projections that alter vesicle transport.
01:43
The Movement of Organelles and Vesicles
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2023
In eukaryotic cells, cytoskeletal filaments such as actin, microtubules, and intermediate filaments form a mesh-like cytoskeletal network. These filaments serve as tracks for transporting cellular cargo. Specialized motor proteins use the chemical energy stored in adenosine triphosphate (ATP) for this transport. During interphase, microtubules are polarized, with the plus-end towards the cell periphery and the minus-end towards the cell center. Two microtubule-associated motor proteins,...
FAQs
Mitochondrial fission and fusion alter the organization and functional state of the mitochondrial network in response to cellular signals. Within organelle remodeling, these opposing processes can influence how cells maintain energy production and coordinate inflammatory signaling. Their relevance in infection research comes from examining how altered mitochondrial dynamics affect host adaptation and whether pathogens exploit those changes.
Lysosomal trafficking and cytoskeletal transport determine how intracellular compartments and their contents are repositioned within the cell. Their coordination helps regulate the delivery of antimicrobial components and supports changes in organelle distribution. Disruption or manipulation of these movements can therefore alter how immune cells process material and direct defensive functions during infection.
Membrane contact formation provides a way for organelles to remain functionally coordinated while their architecture changes. In immunology and infection, these contacts are part of the signaling framework that links organelle activity with inflammatory responses and other cellular adaptations. Studying them helps explain how spatial organization inside the cell contributes to host defense or pathogen-associated dysfunction.
Pathogens can alter organelle dynamics in ways that create replication-supportive intracellular niches or reduce the effectiveness of host defenses. Such manipulation may affect compartment organization, trafficking, or signaling rather than simply damaging the cell. Comparing pathogen-driven changes with regulated host responses helps distinguish protective remodeling from alterations that favor pathogen persistence.
A conceptual analysis begins by asking which organelle property changes: structure, distribution, composition, or activity. Researchers then relate that change to cellular signals and to outcomes such as energy production, inflammatory signaling, antigen processing, or antimicrobial delivery. This framework allows different remodeling events to be compared without treating every intracellular change as the same process.
Changes in organelle remodeling can help connect intracellular organization with major immune-cell functions. Relevant outcomes include altered energy production, inflammatory signaling, antigen processing, and delivery of antimicrobial components. Examining these outcomes together is useful because a structural or trafficking change may have consequences across several defensive activities rather than affecting only one organelle in isolation.
Organelle remodeling links host signaling and intracellular organization to both protective immune functions and pathogen survival strategies. Pathways controlling fission and fusion, lysosomal trafficking, membrane contacts, or cytoskeletal transport may therefore represent points where infection-associated changes can be investigated. Understanding these mechanisms could support efforts to target pathogen-supportive remodeling while preserving host defense.