Silicone Culture Inserts
Silicone Culture Inserts - Related Videos
11:56
Fabrication and Operation of an Oxygen Insert for Adherent Cellular Cultures
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Cited by 1 •
2010
Fabrication and validation of an add-on platform that offers enhanced control over the spatial and temporal oxygenation in a 6-well plate. The device is adaptable to a number of culture systems and can be used to investigate the effects of oxygen on wound healing.
11:29
Development of an Insert Co-culture System of Two Cellular Types in the Absence of Cell-Cell Contact
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Cited by 31 •
2016
In multicellular organisms, secreted soluble factors elicit responses from different cell types as a result of paracrine signaling. Insert co-culture systems offer a simple way to assess the changes mediated by secreted soluble factors in the absence of cell-cell contact.
09:23
A Rapid Filter Insert-based 3D Culture System for Primary Prostate Cell Differentiation
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Cited by 7 •
2017
Here, we present a method for the establishment of a rapid in vitro system that supports the three dimensional culturing and subsequent luminal differentiation of primary prostate epithelial cells.
12:58
Imaging pHluorin-tagged Receptor Insertion to the Plasma Membrane in Primary Cultured Mouse Neurons
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Cited by 8 •
2012
By tagging the extracellular domains of membrane receptors with superecliptic pHluorin, and by imaging these fusion receptors in cultured mouse neurons, we can directly visualize individual vesicular insertion events of the receptors to the plasma membrane. This technique will be instrumental in elucidating the molecular mechanisms governing receptor insertion to the plasma membrane.
06:53
Fabrication and Optimization of Type II Silicon Clathrate Films
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2025
The two-thermal annealing method enables the fabrication of thin films of type II semiconductor silicon clathrate. Post-synthesis treatments, including thermal pressing and reactive ion etching, were performed to improve the material properties. This approach provides an accessible pathway for fabricating tunable clathrate films suitable for next-generation electronic and photonic devices.
FAQs
Silicone culture inserts control where different cell populations begin within the same culture well. The removable barriers maintain defined regions during initial seeding, reducing uncertainty about starting positions and preserving spatial separation when immune and epithelial cells, or other selected populations, are studied together. This organization helps researchers attribute later changes to cell behavior or interactions rather than inconsistent placement.
The cell-free gap provides a common starting geometry for migration measurements. After the barrier is removed, cells can move into the previously unoccupied region, allowing wound-closure or migration assays to compare how quickly and extensively that space becomes covered. Because the gap is established by the insert rather than manual marking, the assay supports more reproducible comparisons across conditions.
Separating populations at the start lets investigators distinguish initial co-culture organization from later contact-dependent responses. In immunology and infection experiments, this can clarify whether immune cells, epithelial cells, or pathogen-exposed cells were positioned in the same region or introduced as distinct groups. The resulting design helps interpret interactions and infection-associated changes within a controlled in vitro setting.
An assay can begin by arranging the silicone insert in a culture well, assigning cells to the defined regions, and maintaining that arrangement during the early culture period. For migration studies, the barrier establishes the cell-free gap before removal; for interaction studies, separate populations can occupy different compartments. Researchers then assess the response after spatial control is released or maintained.
Useful combinations include immune cells with epithelial cells, or cells that have been exposed to a pathogen. The insert allows these populations to be positioned with defined spatial relationships while the assay begins, supporting focused examination of immune-cell interactions, epithelial responses, and infection-related changes. The same format therefore connects cell-behavior measurements with questions about host responses under controlled in vitro conditions.
Readouts can include the extent of cell migration or wound closure, changes in barrier responses, immune-cell interactions, and cellular changes associated with infection. These outcomes can be examined after cells are given a reproducible starting arrangement and, where appropriate, a defined gap. In immunology and infection research, the resulting comparisons help relate spatial organization to host-cell behavior and responses to pathogen exposure.