Gamma-delta T-cell-deficient Mice
Gamma-delta T-cell-deficient Mice - Related Videos
05:47
Intravital Visualization of Gamma Delta Intraepithelial Lymphocytes
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2025
This video demonstrates the visualization of γδ intraepithelial lymphocytes (IELs) in transgenic mice using confocal microscopy. The surgical procedure involves creating a perforation line and making a longitudinal incision to expose the intestinal mucosa and villi. The transgenic mice express reporter proteins exclusively in γδ IELs, allowing their tracking. Intravital imaging captures the dynamic movement of green fluorescence-labeled γδ IELs along the intestinal epithelial cell's basement...
08:10
Isolation and Flow Cytometric Analysis of Human Endocervical Gamma Delta T Cells
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Cited by 4 •
2017
We describe here an isolation method to obtain human endocervical intraepithelial lymphocytes for the analysis of intraepithelial gamma delta T cells. This protocol can be extended for the purification of endocervical gamma delta T cells by magnetic beads or by cell sorting.
11:51
Expansion and Enrichment of Gamma-Delta (γδ) T Cells from Apheresed Human Product
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Cited by 16 •
2021
Presented is a protocol for the expansion of Gamma-Delta (γδ) T cell drug product. Lymphocytes are isolated by elutriation and γδ-enriched with zoledronic acid and interleukin-2. Alpha-beta T cells are depleted using a clinical-grade magnetic separation device. The γδ cells are co-cultured with K562-derived, artificial antigen-presenting cells and expanded.
01:17
The Delta-to-Delta Circuit
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2024
In a delta-delta configuration, the source and the load are connected in a delta manner, forming a closed loop that divides the network into three distinct phases. This configuration makes the phase voltages identical to line voltages. Assuming the sources are in positive sequence, the phase voltages can be expressed directly without having a neutral wire. The phase currents in a delta-connected load are calculated by dividing the phase voltage by the load impedance per phase: In this...
07:55
Establishment and Propagation of Human Retinoblastoma Tumors in Immune Deficient Mice
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Cited by 8 •
2011
A method is described to propagate human retinoblastoma tumors in mice. Tumor cells are directly injected into the eyes of immune deficient mice. Secondary tumors have been successfully established using both cells directly harvested from human tumors and cultured tumorspheres.
FAQs
The Tcrd gene encodes the T-cell receptor component required for γδ T-cell formation. When this gene is disrupted, developing lymphocytes cannot assemble the receptor needed for that lineage, so γδ T cells do not arise. Because αβ T-cell compartments remain largely intact, the model helps separate functions associated with γδ T cells from those performed by αβ T cells.
Maintaining the αβ T-cell compartment reduces a major source of experimental ambiguity. Differences between deficient and control animals can therefore be evaluated in the context of γδ T-cell loss rather than broad elimination of T-cell immunity. This distinction is especially useful when assessing inflammation, cytokine production, adaptive immune responses, or disease severity after infection.
The model can test whether γδ T cells contribute to epithelial surveillance, cytokine production, inflammatory responses, or host defense against bacterial, viral, and parasitic infections. It can also reveal whether these cells influence adaptive immunity. Observing changes in deficient animals compared with controls helps identify functions that depend on γδ T-cell activity.
Researchers compare deficient animals with appropriate control animals and examine how the absence of γδ T cells changes infection or disease outcomes. A reduction in immune defense may indicate a protective role, whereas reduced inflammation or disease severity may suggest that γδ T cells contribute to immunopathology. The same comparison can distinguish pathogen control from harmful host responses.
A typical study compares γδ T-cell-deficient and control animals in an infection or inflammation model, then evaluates the resulting immune and disease responses. Investigators may focus on pathogen-related outcomes, cytokine production, epithelial surveillance, inflammatory changes, or adaptive immunity. Interpreting these measurements together helps connect the missing cell population with specific biological effects.
They are useful when researchers need to isolate the contribution of γδ T cells within a broader immune response. Applications include studying bacterial, viral, or parasitic infection, defining immune regulation at epithelial sites, and evaluating whether γδ T cells shape inflammation or adaptive immunity. The findings can also provide context for immunotherapeutic research involving these functions.