Spheroid Morphology Disruption
Spheroid Morphology Disruption - Related Videos
03:07
Morphological and Physiological Monitoring of Tumor Spheroids Using Optical Coherence Tomography
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2025
Source: Huang, Y. et. al., Longitudinal Morphological and Physiological Monitoring of Three-dimensional Tumor Spheroids Using Optical Coherence Tomography. J. Vis. Exp. (2019)This video demonstrates the use of optical coherence tomography (OCT) to monitor tumor spheroids in a multi-well plate by capturing high-resolution cross-sectional images. By analyzing light reflections from cellular structures, OCT constructs 3D models to assess morphological features like size and shape while also...
06:50
Advancements in the Metabolic Profiling of Three-Dimensional Brain Tumor Spheroids for Drug Screening
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Cited by 1 •
2025
Here, we establish a metabolic profiling platform integrating three-dimensional (3D) glioma spheroids and imaging-based normalization via extracellular flux analysis. This approach enables more accurate assessment of tumor metabolic responses to drug treatments and provides insights into potential mechanisms of resistance.
05:39
Extracellular Flux Assay in 3D Spheroids: A Method for Measuring the Metabolic Activity of Pancreatic Tumor Spheroids
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2023
This protocol describes the technique for performing extracellular flux assay on 3D spheroids generated from the co-culture of pancreatic cancer cells (Patu8902) and activated pancreatic fibroblasts (PS1) to measure the two major energy-producing pathways, glycolysis and mitochondrial respiration.
03:20
Splitting Spheroids for Sub-culturing and Shipping: A Procedure for Propagating and Transferring Spheroids from Small Bowel Neuroendocrine Tumor
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2023
This video demonstrates a strategy to split and propagate spheroids developed from small bowel neuroendocrine tumor cells. These spheroids have small bowel neuroendocrine tumor markers and can be propagated and further used to test anti-cancer drugs.
03:18
Generation of a Three-Dimensional Spheroid Model of Tumor Cell Invasion
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2025
Source: Guyon, J., et al. A 3D Spheroid Model for Glioblastoma. J. Vis. Exp. (2020)The video demonstrates the generation of a three-dimensional spheroid model of tumor cell invasion. Spheroids are formed from human brain tumor cells and embedded in a collagen matrix. The tumor cells at the spheroid periphery adopt an invasive phenotype, secreting proteases to degrade the collagen and advancing to invade the surrounding matrix.
FAQs
Changes in compactness, shape, or structural integrity can indicate that host-pathogen interactions or immune effectors have altered the organization of the cell aggregate. These effects may arise through impaired cell-cell adhesion, reduced viability, disrupted cytoskeletal organization, or changed extracellular matrix interactions. Morphology therefore provides a visible tissue-like readout of injury or remodeling within a multicellular model.
Several linked processes can produce these outcomes. Pathogens or immune effectors may weaken cell-cell adhesion, affect cellular viability, disturb cytoskeletal organization, or interfere with extracellular matrix interactions. The resulting loss of cohesion can make the aggregate irregular or less compact, while more extensive structural injury may lead to fragmentation or collapse. These appearances help connect visible changes with underlying cellular responses.
Morphological changes report organization as well as survival. A spheroid may become irregular, loosen, or lose integrity because cellular adhesion, cytoskeletal structure, or extracellular matrix interactions have changed, even when viability assays provide a different perspective. Measuring both morphology and viability allows researchers to distinguish effects on multicellular architecture from broader changes in cell survival.
Useful features include spheroid size, shape, compactness, and overall structural integrity. Together, these measurements capture different aspects of the response: size reflects aggregate dimensions, shape describes irregularity, compactness indicates cohesion, and integrity summarizes preservation of the three-dimensional structure. Evaluating these features provides a more complete morphological outcome than relying on a single visual characteristic.
Morphological measurements add spatial and structural information that molecular or viability assays may not reveal. They show whether infection, immune activity, or treatment changes the organization of a three-dimensional cell aggregate, while other assays assess survival or molecular responses. Combining these readouts can clarify whether a condition primarily affects tissue-like architecture, cell viability, or both.
Researchers can apply it to examine host-pathogen interactions, immune-mediated injury, and cellular responses to antimicrobial or immunomodulatory treatments. The approach is especially relevant when the experimental goal includes tissue-like organization rather than isolated-cell behavior. Comparing structural outcomes across these conditions can help reveal how infection or inflammation remodels multicellular architecture and whether treatment alters that response.