Sternotomy

Sternotomy is a surgical procedure in which the sternum is divided to provide access to the heart, mediastinum, or other structures within the chest. In a median sternotomy, surgeons make a midline incision and separate the breastbone, then stabilize it with wires or other closure methods after the operation. From an immunology and infection perspective, healing depends on coordinated inflammation, tissue repair, and bone regeneration while the surgical wound remains vulnerable to bacterial contamination. Understanding these processes supports infection prevention, recognition of sternal wound complications, and improved management of mediastinitis, osteomyelitis, and other postoperative infections.

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Research

JoVE Journal - Medicine

Reproducable Paraplegia by Thoracic Aortic Occlusion in a Murine Model of Spinal Cord Ischemia-reperfusion

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Cited by 8 •

2014

The lack of mechanistic understanding of spinal cord ischemia-reperfusion injury has hindered further adjuncts to prevent paraplegia following high risk aortic operations. Thus, the development of animal models is imperative. This manuscript demonstrates reproducible lower extremity paralysis following thoracic aortic occlusion in a murine model.

Technique of Minimally Invasive Transverse Aortic Constriction in Mice for Induction of Left Ventricular Hypertrophy

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Cited by 34 •

2017

The aim of this protocol is to describe step-by-step the technique of minimally invasive transverse aortic constriction (TAC) in mice. By elimination of intubation and ventilation which are mandatory for the commonly used standard procedure, minimally invasive TAC simplifies the operative procedure and reduces the strain put on the animal.

Ex vivo Live Imaging of Lung Metastasis and Their Microenvironment

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Cited by 6 •

2016

We describe a relatively simple method for ex vivo live imaging of the tumor cell-stroma interactions within lung metastasis, utilizing fluorescent reporters in mice. Using spinning-disk confocal microscopy, this technique enables visualization of live cells for at least 4 hr and could be adapted to study other inflammatory lung conditions.

Porcine Heart Excision: A Protocol to Extract Porcine Heart for Cardiac Transplant

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2025

In this video, we describe a step-by-step protocol to extract the heart from a porcine model for cardiac transplantation.

Primary Outcome Assessment in a Pig Model of Acute Myocardial Infarction

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Cited by 13 •

2016

Reliable and accurate outcome assessment is the key for translation of preclinical therapies into clinical treatment. The current paper describes how to assess three clinically relevant primary outcome parameters of cardiac performance and damage in a pig acute myocardial infarction model.

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