Tissue Microarray
Tissue Microarray - Related Videos
09:08
Production of Tissue Microarrays, Immunohistochemistry Staining and Digitalization Within the Human Protein Atlas
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Cited by 202 •
2012
Tissue microarrays allows for an efficient method to gain concurrent information from a multitude of tissues. Representative parts of tissues are assembled into a single paraffin block. Sections from the block are used for immunohistochemistry and analysis of protein expression patterns. Digital scanning generates corresponding images for distribution of data.
08:29
Expression Profiling with Microarrays
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2023
Microarrays are important tools for profiling gene expression, and are based on complementary binding between probes that are attached to glass chips and nucleic acids derived from samples. Using these arrays, scientists can simultaneously evaluate the expression of thousands of genes. In addition, the expression profiles of different cells or tissue types can be compared, allowing researchers to deduce how the expression of different genes change during biological processes, and thus gain...
12:03
Manual Construction of a Tissue Microarray using the Tape Method and a Handheld Microarrayer
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Cited by 5 •
2022
This protocol outlines the tape method on how to manually construct a tissue microarray using FFPE donor blocks of differing depths.
07:04
Performing Custom MicroRNA Microarray Experiments
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Cited by 8 •
2011
A simple procedure of performing custom microRNA microarray experiments is described. The steps include isolating RNA, labeling RNA and reference DNA, hybridizing the samples to microarrays, scanning the microarrays, quantifying and analyzing hybridization signals.
12:30
Glycan Profiling of Plant Cell Wall Polymers using Microarrays
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Cited by 26 •
2012
A technique called Comprehensive Microarray Polymer Profiling (CoMPP) for the characterisation of plant cell wall glycans is described. This method combines the specificity of monoclonal antibodies directed to defined glycan-epitopes with a miniature microarray analytical platform allowing screening of glycan occurrence in a broad range of biological contexts.
FAQs
Parallel processing is the main analytical advantage. Cores from multiple donor tissues are placed in one recipient block, then sectioned together for the same staining or detection workflow. Because specimens share the block and assay conditions, researchers can compare biomarker expression, immune-cell markers, pathogen-associated proteins, or inflammatory responses with greater experimental consistency.
Small cores allow researchers to examine material from many specimens within a single paraffin block while conserving tissue and reagents. This compact format supports side-by-side assessment of diseased, infected, and control samples. It is especially useful when a study needs systematic comparison of immune responses or pathogen-associated signals across a larger specimen set.
The same array format can be sectioned for techniques such as immunohistochemistry or in situ hybridization. This flexibility allows investigators to examine biomarker expression alongside signals relevant to immune cells, pathogens, or inflammation. Selecting the appropriate technique helps align the analysis with the biological feature being compared across the included tissue specimens.
Interpretation depends on how the donor specimens are organized into meaningful comparison groups and on the consistency of the analysis applied to the array sections. Including diseased, infected, and control tissues can clarify differences in marker expression or inflammatory responses. These comparisons support systematic evaluation rather than isolated examination of individual tissue samples.
Researchers first select donor tissues and extract small cores from them. They transfer those cores into a recipient paraffin block, which is then sectioned for downstream analysis. The sections can undergo immunohistochemistry or in situ hybridization to evaluate selected biomarkers, immune-cell markers, pathogen-associated proteins, or inflammatory patterns across the assembled specimens.
This approach is useful when investigators need to compare host responses across infected, diseased, and control tissues in a coordinated experiment. It can reveal patterns in immune-cell markers, pathogen-associated proteins, and inflammation across many specimens. The resulting comparisons help examine host-pathogen interactions and evaluate whether candidate signals remain consistent across different tissue samples.
By testing many tissue specimens under shared analytical conditions, the method helps determine whether a biomarker shows distinguishable expression patterns among relevant sample groups. In immunology and infection research, those groups may reflect disease, infection, or control status. Such systematic evidence can support biomarker validation and contribute to classifying tissues according to their biological or disease-associated profiles.