Bilateral Hindlimb Crush
Bilateral Hindlimb Crush - Related Videos
07:57
Methods for Acute and Subacute Murine Hindlimb Ischemia
0 Views •
Cited by 31 •
2016
Surgical induction of hindlimb ischemia in the mouse is useful to examine angiogenesis, however this is compromised in certain inbred mouse strains that display marked ischemia-induced tissue necrosis. Methods are described to induce subacute limb ischemia using ameroid constrictors to circumvent this problem through the induction of gradual arterial occlusion.
03:37
Performing Crush Axotomy on the Facial Nerve in a Mouse Model
0 Views •
2025
Source: Olmstead, D. N., et al., Facial Nerve Axotomy in Mice: A Model to Study Motoneuron Response to Injury. J. Vis. Exp. (2015).This video demonstrates the facial nerve crush procedure in a mouse, where blunt dissection through key landmarks exposes the facial nerve trunk at the stylomastoid foramen. Forceps are then used to compress the nerve trunk, inducing axonal damage and disrupting neuronal signal transmission.
02:40
Axonal Regeneration in the Sciatic Nerve of a Mouse Following a Crush Injury
0 Views •
2025
Source: Li, Q., et al. Investigating Mammalian Axon Regeneration: In Vivo Electroporation of Adult Mouse Dorsal Root Ganglion. J. Vis. Exp. (2018)This video demonstrates the process of axonal regeneration in a mouse sciatic nerve following a crush injury. The isolated nerve is fixed and prepared for imaging, with fluorescent proteins expressed in neurons to visualize axon growth. Using an epifluorescence microscope, axon regeneration is traced from the injury site to assess the extent of...
08:49
Reproducible Mouse Sciatic Nerve Crush and Subsequent Assessment of Regeneration by Whole Mount Muscle Analysis
0 Views •
Cited by 59 •
2012
In this report we describe a method to crush mouse sciatic nerve. This method uses readily available hemostatic forceps and easily and reproducibly produces complete sciatic nerve crush. In addition, we describe a method to prepare muscle whole mounts suitable for analysis of nerve regeneration after sciatic nerve crush.
09:07
An Optic Nerve Crush Injury Murine Model to Study Retinal Ganglion Cell Survival
0 Views •
Cited by 64 •
2011
This protocol shows how to retrogradely label retinal ganglion cells, and how to subsequently make an optic nerve crush injury in order to analyze retinal ganglion cell survival and apoptosis. It is an experimental disease model for different types of optic neuropathy, including glaucoma.
FAQs
Compression produces injury through simultaneous mechanical and vascular effects. Pressure disrupts cellular integrity in muscle, nerves, blood vessels, and connective tissue, while reduced perfusion limits tissue blood supply. These interacting disturbances allow investigators to examine local damage alongside broader systemic responses, rather than treating muscle injury as an isolated event.
Releasing the compression can introduce ischemia-reperfusion effects, adding a second physiological challenge to the initial mechanical injury. This transition helps researchers evaluate how tissues respond when blood flow conditions change after compression. Considering both phases is important when interpreting vascular dysfunction, cellular damage, inflammation, and subsequent recovery-related findings.
Using both hindlimbs creates a standardized injury configuration across subjects and experiments. This consistency helps researchers compare therapeutic interventions, biomarkers, and functional outcomes under more similar injury conditions. The bilateral design also supports investigation of systemic responses, which may be especially relevant when trauma affects more than one limb and extends beyond a single local tissue site.
The model supports concurrent analysis of inflammation, pain signaling, and tissue repair pathways. These responses connect the initial structural damage with later biological changes involved in recovery. Examining them together can help researchers relate tissue injury to pain-related effects, inflammatory activity, and the mechanisms that support muscle or nerve regeneration.
The essential design feature is controlled mechanical compression applied to both hindlimbs in a consistent manner. Standardizing the bilateral injury improves comparability among experimental groups and strengthens interpretation of treatment effects. After compression and release, investigators can assess tissue damage, systemic responses, biomarkers, functional outcomes, and recovery-related changes using the same injury framework.
These experiments can generate information about tissue damage, vascular dysfunction, inflammation, pain signaling, functional impairment, and repair. Researchers may also measure biomarkers and assess recovery after trauma. Together, these outcomes help evaluate therapeutic interventions and connect observable function with underlying mechanisms of muscle injury, peripheral nerve damage, and regeneration.
The model is relevant when researchers need to study crush syndrome, peripheral nerve and muscle injury, vascular dysfunction, or recovery after trauma. Its value comes from combining injury to several tissue types with potential systemic responses. That breadth allows investigators to test interventions and examine regeneration in a controlled experimental setting that reflects multiple consequences of severe compression.