Fluorescent Laparoscopy
Fluorescent Laparoscopy - Related Videos
08:50
Deep Neuromuscular Blockade Leads to a Larger Intraabdominal Volume During Laparoscopy
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Cited by 49 •
2013
This article presents a method for measuring the working abdominal volume during laproscopic myomectomy using the surgical grasper, and applies this technique to obtain pilot data on whether deep neuromuscular blockade (NMB) can enable the use of lower insufflation pressure. Reduced insufflation pressure during laproscopic surgery has been shown to reduce post-operative pain, and thus the use of NMB during surgery may enable improved patient outcomes.
14:59
Neuronavigation and Laparoscopy Guided Ventriculoperitoneal Shunt Insertion for the Treatment of Hydrocephalus
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Cited by 4 •
2022
Patient outcomes of ventriculoperitoneal (VP) shunt surgery, the mainstay treatment for hydrocephalus in adults, are poor due to high shunt failure rates. We present intraoperative footage of VP shunt insertion using neuronavigation and laparoscopy guidance, with the goal to reduce the risks of proximal and distal shunt catheter failures, respectively.
05:52
Sentinel Lymph Node Mapping and Biopsy for Endometrial Cancer at Early Stage with Laparoscopy
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Cited by 1 •
2021
This protocol describes the identification and resection of sentinel lymph nodes to make the operation as easy and minimally invasive as possible.
05:16
Laparoscopy-endoscopy Cooperative Surgery for the Treatment of Gastric Gastrointestinal Stromal Tumors
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Cited by 2 •
2022
Laparoscopy-endoscopy cooperative surgery is appropriate for the treatment of gastric gastrointestinal stromal tumors less than 5 cm in size. It can achieve the respective advantages of endoscopic and laparoscopic surgeries while avoiding their drawbacks.
09:22
Introduction to Fluorescence Microscopy
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2023
Fluorescence microscopy is a very powerful analytical tool that combines the magnifying properties of light microscopy with visualization of fluorescence. Fluorescence is a phenomenon that involves absorbance and emission of a small range of light wavelengths by a fluorescent molecule known as a fluorophore. Fluorescence microscopy is accomplished in conjunction with the basic light microscope by the addition of a powerful light source, specialized filters, and a means of fluorescently...
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Indocyanine green (ICG) provides the contrast because it is administered and then illuminated with near-infrared light. A specialized laparoscopic camera detects the resulting emitted signal and displays it with the surgical view. This makes selected tissue features distinguishable from surrounding anatomy during the operation, adding information that ordinary white-light visualization may not readily provide.
Near-infrared illumination activates the fluorescent agent so the laparoscopic system can detect its emitted signal. The camera then overlays that signal on the operative image, allowing fluorescence findings and visible anatomy to be considered together. This complementary view helps surgeons interpret structures or tissue features that may be difficult to identify from white-light appearance alone.
The overlay adds contrast without eliminating the conventional surgical view. White light shows the operative anatomy, while fluorescence highlights selected structures or tissue characteristics linked to the administered agent. Using both views supports more informed intraoperative interpretation, because fluorescence supplements visual anatomy rather than serving as the sole basis for surgical assessment.
The approach requires laparoscopic access, administration of a fluorescent agent such as indocyanine green, near-infrared illumination, and a specialized laparoscopic camera capable of detecting emitted fluorescence. The system presents the detected signal together with the surgical image. These components allow the surgeon to inspect fluorescence during the operation rather than relying on a separate postoperative study.
Fluorescence can provide intraoperative information about tissue perfusion, meaning the adequacy of blood supply visible through the detected signal. This gives the surgical team an additional way to evaluate tissue status while operating. The information can support decisions about dissection or the handling of tissue when white-light appearance alone does not provide sufficient guidance.
The technique can highlight bile ducts and lymphatic pathways during laparoscopic surgery, making these anatomical routes easier to recognize within the operative field. Their fluorescence-based visualization may help guide dissection and improve orientation. This is particularly relevant when the surgeon needs to distinguish these pathways from surrounding structures that are less readily apparent under white light.
Fluorescent laparoscopy can help localize selected lesions by displaying fluorescence associated with the administered agent during surgery. It also supports assessment of perfusion and identification of specific anatomical pathways. By adding these findings to the conventional view, the method can inform intraoperative decisions and reduce reliance on visual anatomy alone while the procedure is underway.