In Vivo Bioluminescence Imaging
In Vivo Bioluminescence Imaging - Related Videos
05:06
Bioluminescent Bacterial Imaging In Vivo
0 Views •
Cited by 9 •
2012
This article describes the administration of lux-tagged bacteria to mice and subsequent in vivo analysis using IVIS bioluminescence imaging.
07:33
In vivo Dual Substrate Bioluminescent Imaging
0 Views •
Cited by 20 •
2011
Herein we describe the methods to construct, visualize, and quantify the bioluminescent reactions of both firefly and renilla luciferase enzymes expressed in metastatic breast cancer cells during their growth and metastasis in vivo.
12:19
In vitro and in vivo Bioluminescence Reporter Gene Imaging of Human Embryonic Stem Cells
0 Views •
Cited by 28 •
2008
With the growing interest in stem cell therapies, molecular imaging techniques are ideal for monitoring stem cell behavior after transplantation. Luciferase reporter genes have enabled non-invasive, repetitive assessment of cell survival, location, and proliferation in vivo. This video will demonstrate how to track hESC proliferation in a living mouse.
02:02
Bioluminescence Imaging of Bacterial Infections in Mice
0 Views •
2026
Source: de Vries, C. R., et al., A Delayed Inoculation Model of Chronic Pseudomonas aeruginosa Wound Infection. J. Vis. Exp. (2020).This video demonstrates a bioluminescent imaging technique to monitor bacterial infection progression in a live mouse model. Pathogenic bacteria engineered with the luxABCDE reporter system emit light at the infection site, allowing visualization of metabolically active bacteria. By imaging the mouse over time, researchers can non-invasively track infection...
01:49
In Vivo Bioluminescence Imaging of Human Neural Progenitor Cells in a Mouse Brain
0 Views •
2025
Source: Weber, R. Z., et al., Intracerebral Transplantation and In Vivo Bioluminescence Tracking of Human Neural Progenitor Cells in the Mouse Brain. J. Vis. Exp. (2022)This video demonstrates the method for in vivo bioluminescence imaging of genetically modified human neural progenitor cells injected in a mouse brain. The process involves luciferin injection, imaging preparation, and detection of bioluminescent signals from the transplanted cells at the injection site.
FAQs
The signal depends on the interaction between luciferase and its luciferin substrate. In one configuration, luciferase activity is paired with administered luciferin; in another, organisms express bioluminescent reporter genes that support light production. These alternatives let investigators associate detected emission with biological activity or with the presence and behavior of a labeled biological system in a living subject.
Light produced within the subject must pass through tissue before a sensitive camera captures it. This connects biological activity with an observable optical signal without requiring tissue removal at each observation. Because the camera records emitted photons from the living subject, investigators can follow changes over time and compare patterns during disease progression or treatment response.
Administered luciferin provides the light-producing input for a luciferase system at the time of observation. Reporter-gene strategies instead rely on organisms that express a bioluminescent reporter, linking the signal to the presence or activity of that biological system. The choice therefore affects how researchers associate captured light with the process under study.
Researchers first use a luciferase and luciferin arrangement or a bioluminescent reporter-gene model. For luciferase systems requiring an external substrate, luciferin is administered before observation. A sensitive camera then captures photons passing through tissue, allowing the biological signal to be monitored at successive observations. This sequence supports noninvasive follow-up in living animal models.
The approach can address several medical-model questions without relying on repeated invasive sampling. Investigators can monitor tumor growth, follow infection, examine cell migration, and track gene expression in living animal models. The same approach can also be used during treatment studies, where serial observations reveal whether disease-related activity changes over time and support assessment of treatment response.
Serial observations can show whether the optical signal changes as disease progresses or as treatment is applied. Because the same subject can be followed repeatedly, investigators can compare observations across time rather than depending only on separate endpoint assessments. This supports evaluation of treatment response while reducing invasive sampling and improving the efficiency of animal-model experiments.