Osteomyocutaneous Flap Harvest
Osteomyocutaneous Flap Harvest - Related Videos
07:57
An Experimental Human DIEP Flap Model to Investigate Preservation Strategies for Vascularized Composite Allografts and Free Flaps
0 Views •
2025
This protocol describes the surgical technique for harvesting a human perforator flap model based on the deep inferior epigastric artery pedicle, intended for experimental research.
09:36
Modified Heterotopic Hindlimb Osteomyocutaneous Flap Model in the Rat for Translational Vascularized Composite Allotransplantation Research
0 Views •
Cited by 4 •
2019
Vascularized composite allograft offers life-altering benefits to transplant recipients, but the biological causes of graft rejection and vasculopathy remain poorly understood. The rodent surgical model presented here offers a reproducible, clinically relevant model of transplantation, allowing researchers to evaluate rejection events and potential therapeutic strategies to prevent their occurrence.
08:18
Engineered Vascularized Muscle Flap
0 Views •
Cited by 6 •
2016
To date, thick tissue defects are typically reconstructed by applying autologous tissue flaps or engineered tissues. In this protocol, we present a new method for engineering vascularized tissue flap bearing an autologous pedicle, to serve as a substitute to autologous flaps.
06:32
Coronoid-Temporalis Pedicled Flap for Orbital Floor Defect Reconstruction
0 Views •
2025
This protocol presents a novel coronoid-temporalis pedicled flap (CTPF) technique for reconstructing small-to-medium orbital floor defects. CTPF avoids microvascular risks while maintaining biomechanical stability. Clinical cases demonstrate effective structural restoration, reduced complications, and preserved facial symmetry, offering a viable alternative to traditional grafts or alloplastic materials.
02:29
Harvesting an Inguinal Draining Lymph Node from a Murine Model
0 Views •
2025
This video demonstrates the surgical isolation procedure of the inguinal draining lymph node from a mouse model. Incision of the abdominal region provides access to the inguinal lymph node, which is surgically harvested. The harvested translucent inguinal lymph node can be used for immunological studies.
FAQs
The vascular pedicle carries the blood vessels that supply the harvested bone, muscle, and skin. Keeping this connection intact during tissue isolation helps maintain perfusion until the flap reaches the recipient site. Once transferred, microsurgical reconnection of those vessels supports continued blood flow, allowing the composite tissue to remain viable while contributing to reconstruction and wound healing.
Each tissue component addresses a different structural need within a complex defect. Bone provides skeletal support, muscle contributes soft-tissue volume, and skin supplies external coverage. Combining them in one flap allows surgeons to address hard- and soft-tissue loss together rather than treating these problems as entirely separate reconstructive tasks, supporting restoration of form and function.
Microsurgery reconnects the flap’s blood vessels with vessels at the recipient site. This vascular connection restores circulation to the transplanted tissues after transfer, which is essential for maintaining perfusion of the bone, muscle, and skin. The technique therefore links successful tissue harvesting with the flap’s ability to participate in healing and reconstruction at its new location.
The process begins by isolating the required composite tissue while preserving its vascular pedicle. The flap is then transferred to the defect, where its supplying vessels are reconnected to recipient vessels using microsurgery. This sequence preserves the relationship between the tissue components and their blood supply while positioning the flap for three-dimensional reconstruction.
This approach is relevant when a defect includes both skeletal and soft-tissue loss, particularly after trauma, tumor resection, or infection. Because the flap combines structural tissue with muscle and skin, it can address several dimensions of the defect in one reconstructive strategy. Its use is therefore tied to the need for integrated coverage and support.
By supplying skeletal support together with durable soft-tissue coverage, the reconstruction can help restore form and function in complex defects. These features also support wound healing and subsequent rehabilitation. The intended result extends beyond closing the wound, because rebuilding both structural and soft-tissue elements may help the affected region progress toward functional recovery.