Mouse Spine Stabilizer
Mouse Spine Stabilizer - Related Videos
05:28
A Mouse Model of Lumbar Spine Instability
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Cited by 14 •
2021
We developed a lumbar intervertebral disc degeneration mouse model by resection of L3–L5 spinous processes along with supra- and inter-spinous ligaments and detachment of paraspinous muscles.
06:30
Visualization and Genetic Manipulation of Dendrites and Spines in the Mouse Cerebral Cortex and Hippocampus using In utero Electroporation
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Cited by 27 •
2012
This article describes in detail a protocol to electroporate in utero the cerebral cortex and the hippocampus at E14.5 in mice. We also show that this is a valuable method to study dendrites and spines in these two cerebral regions.
09:53
Two-Photon in vivo Imaging of Dendritic Spines in the Mouse Cortex Using a Thinned-skull Preparation
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Cited by 19 •
2014
Time-lapse imaging in the living animal provides valuable information on structural reorganization in the intact brain. Here, we introduce a thinned-skull preparation that allows transcranial imaging of fluorescently labeled synaptic structures in the living mouse cortex by two-photon microscopy.
04:36
Surgical Induction of Lumbar Spine Instability in Mouse Model: A Surgical Procedure for the Resection of the Lumbar Spinous Processes and Connected Ligaments in Murine Model
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2025
In this video, we describe the surgical procedure to induce lumbar spine instability in a mouse model by resecting the Lumbar L3 to L5 spinous process along with supraspinous and interspinous ligaments.
02:49
Harvesting DRG from Rat Spine: A Procedure to Extract Dorsal Root Ganglion from the Spine of a Rat Model
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2023
In this video, we demonstrate the extraction of dorsal root ganglion or DRG from a harvested rat spine for downstream applications.
FAQs
Supports or clamps attached to a rigid frame constrain the vertebral column so it does not shift during an intervention. This mechanical restraint preserves a consistent relationship between the spine and the instrument or imaging field, while the frame arrangement still leaves the operative area accessible. The result is less motion-related variability in observations and measurements.
The main experimental value is positional consistency. If animals are held in different spinal orientations or allowed to move, surgical access, imaging views, injections, and recordings may not be directly comparable. Stabilization reduces that source of variation, helping investigators distinguish effects associated with the intervention from differences caused by changing vertebral position.
Its supports or clamps must restrict vertebral movement without obstructing the surgical field. The rigid frame provides a stable foundation, while the placement of spinal restraints determines how effectively motion is limited and how easily researchers can reach the target area. This balance is important because excessive obstruction could interfere with procedures, whereas insufficient restraint may increase measurement variability.
A typical workflow places the mouse so the vertebral column is aligned with the experimental field, secures the spine to the rigid frame using the available supports or clamps, and then performs the planned surgery, imaging, recording, or injection. Researchers maintain that position throughout the procedure to reduce motion and support consistent access and measurements.
The apparatus can support spinal surgery, imaging, spinal cord injury modeling, electrophysiological recordings, and injections. Across these procedures, keeping the vertebral column steady helps preserve the intended experimental position and reduces movement-related error. That consistency is particularly useful when researchers compare interventions, measurements, or outcomes across multiple animals.
In preclinical medicine, consistent spinal positioning strengthens experimental models used to study spinal trauma, neural repair, pain, and therapeutic development. Stable positioning helps researchers compare animals more reliably and interpret procedure-related measurements with fewer differences caused by movement. It therefore supports clearer evaluation of experimental interventions and their outcomes in controlled laboratory studies.